IL7-hCD25 and IL7-Cre BAC transgenic mouse lines: new tools for analysis of IL-7 expressing cells

John F Repass, Micheline N Laurent, Carla Carter, Boris Reizis, Mark T Bedford, Kim Cardenas, Priyanka Narang, Mark Coles, Ellen R Richie

Research output: Contribution to journalArticlepeer-review


IL-7 is a cytokine that is required for T-cell development and homeostasis as well as for lymph node organogenesis. Despite the importance of IL-7 in the immune system and its potential therapeutic relevance, questions remain regarding the sites of IL-7 synthesis, specific cell types involved and molecular mechanisms regulating IL-7 expression. To address these issues, we generated two bacterial artificial chromosome (BAC) transgenic mouse lines in which IL-7 regulatory elements drive expression of either Cre recombinase or a human CD25 (hCD25) cell surface reporter molecule. Expression of the IL-7.hCD25 BAC transgene, detected by reactivity with anti-hCD25 antibody, mimicked endogenous IL-7 expression. Fetal and adult tissues from crosses between IL-7.Cre transgenic mice and Rosa26R or R26-EYFP reporters demonstrated X-gal or YFP staining in tissues known to express endogenous IL-7 at some stage during development. These transgenic lines provide novel genetic tools to identify IL-7 producing cells in various tissues and to manipulate gene expression selectively in IL-7 expressing cells.
Original languageEnglish
Pages (from-to)281-7
Number of pages7
JournalGenesis (New York, N.Y. : 2000)
Issue number4
Publication statusPublished - 2009


  • Animals
  • Animals, Newborn
  • Chromosomes, Artificial, Bacterial
  • Female
  • Flow Cytometry
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental
  • Humans
  • Immunohistochemistry
  • Integrases
  • Interleukin-2 Receptor alpha Subunit
  • Interleukin-7
  • Male
  • Mice
  • Mice, Transgenic
  • Promoter Regions, Genetic
  • Recombinant Fusion Proteins
  • Reverse Transcriptase Polymerase Chain Reaction
  • Thymus Gland

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