By the same authors

From the same journal

From the same journal

In vivo architecture and action of bacterial structural maintenance of chromosome proteins

Research output: Contribution to journalArticle

Author(s)

  • Anjana Badrinarayanan
  • Rodrigo Reyes-Lamothe
  • Stephan Uphoff
  • Mark C Leake
  • David J Sherratt

Department/unit(s)

Publication details

JournalScience
DatePublished - 26 Oct 2012
Issue number6106
Volume338
Number of pages4
Pages (from-to)528-531
Original languageEnglish

Abstract

SMC (structural maintenance of chromosome) proteins act ubiquitously in chromosome processing. In Escherichia coli, the SMC complex MukBEF plays roles in chromosome segregation and organization. We used single-molecule millisecond multicolor fluorescence microscopy of live bacteria to reveal that a dimer of dimeric fluorescent MukBEF molecules acts as the minimal functional unit. On average, 8 to 10 of these complexes accumulated as "spots" in one to three discrete chromosome-associated regions of the cell, where they formed higher-order structures. Functional MukBEF within spots exchanged with freely diffusing complexes at a rate of one complex about every 50 seconds in reactions requiring adenosine triphosphate (ATP) hydrolysis. Thus, by functioning in pairs, MukBEF complexes may undergo multiple cycles of ATP hydrolysis without being released from DNA, analogous to the behavior of well-characterized molecular motors.

Discover related content

Find related publications, people, projects, datasets and more using interactive charts.

View graph of relations