In vivo biocompatibility of custom-fabricated apatite-wollastonite-mesenchymal stromal cell constructs

TY - JOUR

T1 - In vivo biocompatibility of custom-fabricated apatite-wollastonite-mesenchymal stromal cell constructs

AU - Lee, Jennifer A

AU - Knight, Charlotte A

AU - Kun, Xiao

AU - Yang, Xuebin B

AU - Wood, David J

AU - Dalgarno, Kenneth W

AU - Genever, Paul G

N1 - © 2015 Wiley Periodicals, Inc.

PY - 2015

Y1 - 2015

N2 - We have used the additive manufacturing technology of selective laser sintering (SLS), together with post SLS heat treatment, to produce porous three dimensional scaffolds from the glass-ceramic apatite-wollastonite (A-W). The A-W scaffolds were custom-designed to incorporate a cylindrical central channel to increase cell penetration and medium flow to the center of the scaffolds under dynamic culture conditions during in vitro testing and subsequent in vivo implantation. The scaffolds were seeded with human bone marrow mesenchymal stromal cells (MSCs) and cultured in spinner flasks. Using confocal and scanning electron microscopy, we demonstrated that MSCs formed and maintained a confluent layer of viable cells on all surfaces of the A-W scaffolds during dynamic culture. MSC-seeded, with and without osteogenic pre-differentiation, and unseeded A-W scaffolds were implanted subcutaneously in MF1 nude mice where osteoid formation and tissue in-growth were observed following histological assessment. The results demonstrate that the in vivo biocompatibility and osteo-supportive capacity of A-W scaffolds can be enhanced by SLS-custom design, without the requirement for osteogenic pre-induction, to advance their potential as patient-specific bone replacement materials. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2015.

AB - We have used the additive manufacturing technology of selective laser sintering (SLS), together with post SLS heat treatment, to produce porous three dimensional scaffolds from the glass-ceramic apatite-wollastonite (A-W). The A-W scaffolds were custom-designed to incorporate a cylindrical central channel to increase cell penetration and medium flow to the center of the scaffolds under dynamic culture conditions during in vitro testing and subsequent in vivo implantation. The scaffolds were seeded with human bone marrow mesenchymal stromal cells (MSCs) and cultured in spinner flasks. Using confocal and scanning electron microscopy, we demonstrated that MSCs formed and maintained a confluent layer of viable cells on all surfaces of the A-W scaffolds during dynamic culture. MSC-seeded, with and without osteogenic pre-differentiation, and unseeded A-W scaffolds were implanted subcutaneously in MF1 nude mice where osteoid formation and tissue in-growth were observed following histological assessment. The results demonstrate that the in vivo biocompatibility and osteo-supportive capacity of A-W scaffolds can be enhanced by SLS-custom design, without the requirement for osteogenic pre-induction, to advance their potential as patient-specific bone replacement materials. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2015.

U2 - 10.1002/jbm.a.35448

DO - 10.1002/jbm.a.35448

M3 - Article

C2 - 25777813

SN - 1549-3296

VL - 103

SP - 3188

EP - 3200

JO - Journal of biomedical materials research part a

JF - Journal of biomedical materials research part a

IS - 10

ER -