Projects per year
Abstract
Probing the detailed interaction between lytic polysaccharide monooxygenases (LPMOs) and their polysaccharide substrates is key to revealing further insights into the mechanism of action of this class of enzymes on recalcitrant biomass. This investigation is somewhat hindered, however, by the insoluble nature of the substrates, which precludes the use of most optical spectroscopic techniques. Herein, we report a new semi-oriented EPR method which evaluates directly the binding of cellulose-active LPMOs to crystalline cellulose. We make use of the intrinsic order of cellulose fibres in Apium graveolens (celery) to orient the LPMO with respect to the magnetic field of an EPR spectrometer. The subsequent angle-dependent changes observed in the EPR spectra can then be related to the orientation of the g matrix principal directions with respect to the magnetic field of the spectrometer and, hence, to the binding of the enzyme onto the cellulose fibres. This method, which does not require specific modification of standard CW-EPR equipment, can be used as a general procedure to investigate LPMO–cellulose interactions.
Original language | English |
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Pages (from-to) | 3413-3422 |
Number of pages | 9 |
Journal | Dalton Transactions |
Volume | 49 |
DOIs | |
Publication status | Published - 3 Mar 2020 |
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Mechanistic insights into lytic polysaccharide monooxygenases
BBSRC (BIOTECHNOLOGY AND BIOLOGICAL SCIENCES RESEARCH COUNCIL)
1/04/18 → 31/03/23
Project: Research project (funded) › Research
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Learning from marine wood borers; enzymes and mechanisms of lignocellulose digestion
McQueen Mason, S. J., Bruce, N. C., Davies, G. J. & Walton, P. H.
BBSRC (BIOTECHNOLOGY AND BIOLOGICAL SCIENCES RESEARCH COUNCIL)
1/04/14 → 31/03/19
Project: Research project (funded) › Research