Integrin receptor involvement in actin cable formation in an in vitro model of events associated with wound contraction

P Stephens; P G Genever; E J Wood; M J Raxworthy

doi:10.1016/S1357-2725(96)00123-9

Integrin receptor involvement in actin cable formation in an in vitro model of events associated with wound contraction

P Stephens, P G Genever, E J Wood, M J Raxworthy

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Actin cables have been reported to act in vivo as contractile 'purse strings' capable of closing embryonic wounds through generation of circumferential tension. Furthermore, their involvement in wounds within in vitro model systems suggests that actin cable contraction may be an important mechanism involved in the process of wound closure. The aim of this study therefore, was to investigate the appearance of actin cables in a contracting fibroblast populated collagen lattice, an in vitro model of events associated with wound contraction. Utilising this in vitro model, the time-course of actin cable production was investigated and the involvement of integrin receptors analysed using immunofluorescent labelling techniques. Over a period of hours distinct cellular cable-like structures developed at the edges of collagen lattices coinciding with the onset of contraction. Cellular organisation within the cable was evident as was polymerisation of actin microfilaments into elongated stress fibres forming a continuous cell-cell 'actin cable' around the circumference of the lattice. Immunolocalisation demonstrated that integrin receptor subunit beta(1) and alpha(2) but not alpha(5) were involved in apparent intimate cell-cell contact between juxtaposed fibroblasts within this actin cable. This study demonstrates the involvement of integrin receptors in actin cable formation within collagen lattice systems undergoing reorganisation. Such integrin involvement may enable participating cells to respond to the tensional status of their surrounding environment and via cell-cell communication, to permit a co-ordinated contraction of the cable. It is concluded that integrin receptor involvement in active actin cable contraction may be involved in the process of wound contraction. (C) 1997 Elsevier Science Ltd.

Original language	English
Pages (from-to)	121-128
Number of pages	8
Journal	International Journal of Biochemistry and Cell Biology
Volume	29
Issue number	1
DOIs	https://doi.org/10.1016/S1357-2725(96)00123-9
Publication status	Published - Jan 1997

Keywords

actin cable
fibroblast populated collagen lattice
contraction
integrin receptors
cell-cell communication
CAPILLARY ENDOTHELIAL-CELLS
SMOOTH MUSCLE ACTIN
COLLAGEN LATTICES
FIBROBLAST CONTRACTION
GRANULATION TISSUE
INVITRO
GROWTH
GELS
MYOFIBROBLASTS
REORGANIZATION

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10.1016/S1357-2725(96)00123-9

Cite this

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title = "Integrin receptor involvement in actin cable formation in an in vitro model of events associated with wound contraction",

abstract = "Actin cables have been reported to act in vivo as contractile 'purse strings' capable of closing embryonic wounds through generation of circumferential tension. Furthermore, their involvement in wounds within in vitro model systems suggests that actin cable contraction may be an important mechanism involved in the process of wound closure. The aim of this study therefore, was to investigate the appearance of actin cables in a contracting fibroblast populated collagen lattice, an in vitro model of events associated with wound contraction. Utilising this in vitro model, the time-course of actin cable production was investigated and the involvement of integrin receptors analysed using immunofluorescent labelling techniques. Over a period of hours distinct cellular cable-like structures developed at the edges of collagen lattices coinciding with the onset of contraction. Cellular organisation within the cable was evident as was polymerisation of actin microfilaments into elongated stress fibres forming a continuous cell-cell 'actin cable' around the circumference of the lattice. Immunolocalisation demonstrated that integrin receptor subunit beta(1) and alpha(2) but not alpha(5) were involved in apparent intimate cell-cell contact between juxtaposed fibroblasts within this actin cable. This study demonstrates the involvement of integrin receptors in actin cable formation within collagen lattice systems undergoing reorganisation. Such integrin involvement may enable participating cells to respond to the tensional status of their surrounding environment and via cell-cell communication, to permit a co-ordinated contraction of the cable. It is concluded that integrin receptor involvement in active actin cable contraction may be involved in the process of wound contraction. (C) 1997 Elsevier Science Ltd.",

keywords = "actin cable, fibroblast populated collagen lattice, contraction, integrin receptors, cell-cell communication, CAPILLARY ENDOTHELIAL-CELLS, SMOOTH MUSCLE ACTIN, COLLAGEN LATTICES, FIBROBLAST CONTRACTION, GRANULATION TISSUE, INVITRO, GROWTH, GELS, MYOFIBROBLASTS, REORGANIZATION",

author = "P Stephens and Genever, {P G} and Wood, {E J} and Raxworthy, {M J}",

year = "1997",

month = jan,

doi = "10.1016/S1357-2725(96)00123-9",

language = "English",

volume = "29",

pages = "121--128",

journal = "International Journal of Biochemistry and Cell Biology",

issn = "1357-2725",

publisher = "Elsevier",

number = "1",

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TY - JOUR

T1 - Integrin receptor involvement in actin cable formation in an in vitro model of events associated with wound contraction

AU - Stephens, P

AU - Genever, P G

AU - Wood, E J

AU - Raxworthy, M J

PY - 1997/1

Y1 - 1997/1

N2 - Actin cables have been reported to act in vivo as contractile 'purse strings' capable of closing embryonic wounds through generation of circumferential tension. Furthermore, their involvement in wounds within in vitro model systems suggests that actin cable contraction may be an important mechanism involved in the process of wound closure. The aim of this study therefore, was to investigate the appearance of actin cables in a contracting fibroblast populated collagen lattice, an in vitro model of events associated with wound contraction. Utilising this in vitro model, the time-course of actin cable production was investigated and the involvement of integrin receptors analysed using immunofluorescent labelling techniques. Over a period of hours distinct cellular cable-like structures developed at the edges of collagen lattices coinciding with the onset of contraction. Cellular organisation within the cable was evident as was polymerisation of actin microfilaments into elongated stress fibres forming a continuous cell-cell 'actin cable' around the circumference of the lattice. Immunolocalisation demonstrated that integrin receptor subunit beta(1) and alpha(2) but not alpha(5) were involved in apparent intimate cell-cell contact between juxtaposed fibroblasts within this actin cable. This study demonstrates the involvement of integrin receptors in actin cable formation within collagen lattice systems undergoing reorganisation. Such integrin involvement may enable participating cells to respond to the tensional status of their surrounding environment and via cell-cell communication, to permit a co-ordinated contraction of the cable. It is concluded that integrin receptor involvement in active actin cable contraction may be involved in the process of wound contraction. (C) 1997 Elsevier Science Ltd.

AB - Actin cables have been reported to act in vivo as contractile 'purse strings' capable of closing embryonic wounds through generation of circumferential tension. Furthermore, their involvement in wounds within in vitro model systems suggests that actin cable contraction may be an important mechanism involved in the process of wound closure. The aim of this study therefore, was to investigate the appearance of actin cables in a contracting fibroblast populated collagen lattice, an in vitro model of events associated with wound contraction. Utilising this in vitro model, the time-course of actin cable production was investigated and the involvement of integrin receptors analysed using immunofluorescent labelling techniques. Over a period of hours distinct cellular cable-like structures developed at the edges of collagen lattices coinciding with the onset of contraction. Cellular organisation within the cable was evident as was polymerisation of actin microfilaments into elongated stress fibres forming a continuous cell-cell 'actin cable' around the circumference of the lattice. Immunolocalisation demonstrated that integrin receptor subunit beta(1) and alpha(2) but not alpha(5) were involved in apparent intimate cell-cell contact between juxtaposed fibroblasts within this actin cable. This study demonstrates the involvement of integrin receptors in actin cable formation within collagen lattice systems undergoing reorganisation. Such integrin involvement may enable participating cells to respond to the tensional status of their surrounding environment and via cell-cell communication, to permit a co-ordinated contraction of the cable. It is concluded that integrin receptor involvement in active actin cable contraction may be involved in the process of wound contraction. (C) 1997 Elsevier Science Ltd.

KW - actin cable

KW - fibroblast populated collagen lattice

KW - contraction

KW - integrin receptors

KW - cell-cell communication

KW - CAPILLARY ENDOTHELIAL-CELLS

KW - SMOOTH MUSCLE ACTIN

KW - COLLAGEN LATTICES

KW - FIBROBLAST CONTRACTION

KW - GRANULATION TISSUE

KW - INVITRO

KW - GROWTH

KW - GELS

KW - MYOFIBROBLASTS

KW - REORGANIZATION

U2 - 10.1016/S1357-2725(96)00123-9

DO - 10.1016/S1357-2725(96)00123-9

M3 - Article

SN - 1357-2725

VL - 29

SP - 121

EP - 128

JO - International Journal of Biochemistry and Cell Biology

JF - International Journal of Biochemistry and Cell Biology

IS - 1

ER -