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Inter-individual variation in urothelial DNA repair gene expression in vitro

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Inter-individual variation in urothelial DNA repair gene expression in vitro. / Crallan, R A; Lord, P G; Rees, R W; Southgate, J.

In: Toxicology in vitro, Vol. 16, No. 4, 08.2002, p. 383-387.

Research output: Contribution to journal › Article › peer-review

Harvard

Crallan, RA, Lord, PG, Rees, RW & Southgate, J 2002, 'Inter-individual variation in urothelial DNA repair gene expression in vitro', Toxicology in vitro, vol. 16, no. 4, pp. 383-387.

APA

Crallan, R. A., Lord, P. G., Rees, R. W., & Southgate, J. (2002). Inter-individual variation in urothelial DNA repair gene expression in vitro. Toxicology in vitro, 16(4), 383-387.

Vancouver

Crallan RA, Lord PG, Rees RW, Southgate J. Inter-individual variation in urothelial DNA repair gene expression in vitro. Toxicology in vitro. 2002 Aug;16(4):383-387.

Author

Crallan, R A ; Lord, P G ; Rees, R W ; Southgate, J. / Inter-individual variation in urothelial DNA repair gene expression in vitro. In: Toxicology in vitro. 2002 ; Vol. 16, No. 4. pp. 383-387.

Bibtex - Download

@article{336cc20d0f304e2abef2c31444bb34cf,
title = "Inter-individual variation in urothelial DNA repair gene expression in vitro",
abstract = "DNA repair efficiency may play a significant role in individual susceptibility to bladder cancer, the third most common cancer in Europe. Bladder cancer arises from the urothelial cell layer which lines the urinary tract. As DNA repair gene expression levels should reflect DNA repair capacity, we investigated the expression of genes from the base excision, nucleotide excision and mismatch repair pathways in normal human urothelial (NHU) cells in vitro. RNA was extracted from six independent NHU cell lines and expression of 26 DNA repair genes was determined by ribonuclease protection assay. The results show that all the genes analysed were detected in NHU cells in vitro with a similar expression pattern in most cell lines. However, there was some variation between cell lines, with one expressing base excision repair genes very strongly, but another having weak expression of mismatch repair genes. These results suggest that DNA repair genes are constitutively expressed by NHU cells and that there is some inter-individual variation. Prospective studies are required to determine whether these differences in gene expression may play a role in susceptibility to bladder cancer. (C) 2002 Elsevier Science Ltd. All rights reserved.",
keywords = "urothelium, bladder cancer, cancer susceptibility, DNA repair, gene expression, ribonuclease protection assay, LUNG-CANCER, REDUCED EXPRESSION, BLADDER-CANCER, RISK, POLYMORPHISMS, CELLS, ADDUCTS, XRCC1",
author = "Crallan, {R A} and Lord, {P G} and Rees, {R W} and J Southgate",
year = "2002",
month = aug,
language = "English",
volume = "16",
pages = "383--387",
journal = "Toxicology in vitro",
issn = "0887-2333",
publisher = "Elsevier",
number = "4",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Inter-individual variation in urothelial DNA repair gene expression in vitro

AU - Crallan, R A

AU - Lord, P G

AU - Rees, R W

AU - Southgate, J

PY - 2002/8

Y1 - 2002/8

N2 - DNA repair efficiency may play a significant role in individual susceptibility to bladder cancer, the third most common cancer in Europe. Bladder cancer arises from the urothelial cell layer which lines the urinary tract. As DNA repair gene expression levels should reflect DNA repair capacity, we investigated the expression of genes from the base excision, nucleotide excision and mismatch repair pathways in normal human urothelial (NHU) cells in vitro. RNA was extracted from six independent NHU cell lines and expression of 26 DNA repair genes was determined by ribonuclease protection assay. The results show that all the genes analysed were detected in NHU cells in vitro with a similar expression pattern in most cell lines. However, there was some variation between cell lines, with one expressing base excision repair genes very strongly, but another having weak expression of mismatch repair genes. These results suggest that DNA repair genes are constitutively expressed by NHU cells and that there is some inter-individual variation. Prospective studies are required to determine whether these differences in gene expression may play a role in susceptibility to bladder cancer. (C) 2002 Elsevier Science Ltd. All rights reserved.

AB - DNA repair efficiency may play a significant role in individual susceptibility to bladder cancer, the third most common cancer in Europe. Bladder cancer arises from the urothelial cell layer which lines the urinary tract. As DNA repair gene expression levels should reflect DNA repair capacity, we investigated the expression of genes from the base excision, nucleotide excision and mismatch repair pathways in normal human urothelial (NHU) cells in vitro. RNA was extracted from six independent NHU cell lines and expression of 26 DNA repair genes was determined by ribonuclease protection assay. The results show that all the genes analysed were detected in NHU cells in vitro with a similar expression pattern in most cell lines. However, there was some variation between cell lines, with one expressing base excision repair genes very strongly, but another having weak expression of mismatch repair genes. These results suggest that DNA repair genes are constitutively expressed by NHU cells and that there is some inter-individual variation. Prospective studies are required to determine whether these differences in gene expression may play a role in susceptibility to bladder cancer. (C) 2002 Elsevier Science Ltd. All rights reserved.

KW - urothelium

KW - bladder cancer

KW - cancer susceptibility

KW - DNA repair

KW - gene expression

KW - ribonuclease protection assay

KW - LUNG-CANCER

KW - REDUCED EXPRESSION

KW - BLADDER-CANCER

KW - RISK

KW - POLYMORPHISMS

KW - CELLS

KW - ADDUCTS

KW - XRCC1

M3 - Article

VL - 16

SP - 383

EP - 387

JO - Toxicology in vitro

JF - Toxicology in vitro

SN - 0887-2333

IS - 4

ER -

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