TY - JOUR
T1 - Interleukins 4 and 13 upregulate expression of CD44 in human colonic epithelial cell lines
AU - Trejdosiewicz, Ludwik K.
AU - Morton, Ruth
AU - Yang, Yaoquin
AU - Banks, Roz E.
AU - Selby, Peter J.
AU - Southgate, Jennifer
PY - 1998/10/1
Y1 - 1998/10/1
N2 - Interleukin 4 (IL-4) inhibits carcinoma cell growth and promotes expression of differentiation-associated products by normal and malignant epithelial cells. The effects of IL-4 and IL-13 on expression of the CD44 transmembrane adhesion receptor were examined in human epithelial cell lines of colonic (HT-29, CaCo-2, DLD-1, T84), breast (MCF-7, ZR75-1) and liver (Hep-G2, PLC/PRF/5) origins as well as mitogen-activated and resting peripheral blood lymphocytes (PBL) and T cell lines (Jurkat, HUT78). Liver and Jurkat cells were negative for CD44, Colonic, breast and HUT78 cells expressed CD44 constitutively and all except DLD-1 and HUT78 also expressed CD44 splice variant (CD44v) epitopes. All cell lines expressed IL-4 receptors, but IL-4 and IL-13 induced upregulation of CD44 only in the colonic cell lines. CD44v was also upregulated, but there was no de novo induction of CD44v in variant-negative cells and no de novo expression of CD44 in the CD44- lines. CD44 upregulation in mitogen-activated PBL was not increased by IL-4 and IL-13 and was not inhibited by neutralizing antibodies. Other cytokines tested [interferon γ (IFN-γ, tumour necrosis factor α (TNF-α), transforming growth factor β1 (TGF-β1) and IL-6] did not affect CD44 core epitope expression in the cell lines tested.
AB - Interleukin 4 (IL-4) inhibits carcinoma cell growth and promotes expression of differentiation-associated products by normal and malignant epithelial cells. The effects of IL-4 and IL-13 on expression of the CD44 transmembrane adhesion receptor were examined in human epithelial cell lines of colonic (HT-29, CaCo-2, DLD-1, T84), breast (MCF-7, ZR75-1) and liver (Hep-G2, PLC/PRF/5) origins as well as mitogen-activated and resting peripheral blood lymphocytes (PBL) and T cell lines (Jurkat, HUT78). Liver and Jurkat cells were negative for CD44, Colonic, breast and HUT78 cells expressed CD44 constitutively and all except DLD-1 and HUT78 also expressed CD44 splice variant (CD44v) epitopes. All cell lines expressed IL-4 receptors, but IL-4 and IL-13 induced upregulation of CD44 only in the colonic cell lines. CD44v was also upregulated, but there was no de novo induction of CD44v in variant-negative cells and no de novo expression of CD44 in the CD44- lines. CD44 upregulation in mitogen-activated PBL was not increased by IL-4 and IL-13 and was not inhibited by neutralizing antibodies. Other cytokines tested [interferon γ (IFN-γ, tumour necrosis factor α (TNF-α), transforming growth factor β1 (TGF-β1) and IL-6] did not affect CD44 core epitope expression in the cell lines tested.
KW - CD44
KW - Colon
KW - IL-13
KW - IL-4
UR - http://www.scopus.com/inward/record.url?scp=0032190879&partnerID=8YFLogxK
U2 - 10.1006/cyto.1998.0361
DO - 10.1006/cyto.1998.0361
M3 - Article
C2 - 9811528
AN - SCOPUS:0032190879
SN - 1043-4666
VL - 10
SP - 756
EP - 765
JO - Cytokine
JF - Cytokine
IS - 10
ER -