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From the same journal

Inverse relationship between the expression of the human papillomavirus type 16 transcription factor E2 and virus DNA copy number during the progression of cervical intraepithelial neoplasia

Research output: Contribution to journalArticle

Author(s)

  • M Stevenson
  • L C Hudson
  • J E Burns
  • R L Stewart
  • M Wells
  • N J Maitland

Department/unit(s)

Publication details

JournalJournal of general virology
DatePublished - Jul 2000
Volume81
Number of pages8
Pages (from-to)1825-1832
Original languageEnglish

Abstract

The human papillomavirus type 16 (HPV-16) status of 43 cervical biopsies, which had been characterized histologically as normal, various grades of cervical intraepithelial neoplasia (CIN) and invasive squamous cell carcinoma, was examined by using (i) a novel antibody against the HPV-16 E2 protein, (ii) sensitive HPV-16 DNA in situ hybridization and (iii) microdissection/PCR for the E2 ORF, The data indicate that E2 protein expression is highest in koilocytes in lower-grade CIN (I), but decreases with increasing grade, whereas the detection of HPV DNA is delayed until CIN I/II, rising to the highest levels in carcinoma cells. Go-localization of E2 with HPV-16 DNA-positive cells was most commonly observed in koilocytes in CIN II lesions. PGR analyses of microdissected epithelium from the same or serial sections indicated that E2 ORFs were retained in an intact form in a number of higher-grade GIN lesions and invasive carcinomas.

    Research areas

  • PROTEIN, REPLICATION, CARCINOMA, CANCERS, CELLS, TRANSACTIVATION, SEQUENCES, HPV-16, ORIGIN, GRADE

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