Investigating cellular recognition using crispr/cas9 genetic screening.

Zheng-Shan Chong; Gavin J Wright; Sumana Sharma

doi:10.1016/j.tcb.2020.05.005

Investigating cellular recognition using crispr/cas9 genetic screening.

Zheng-Shan Chong, Gavin J Wright, Sumana Sharma

Research output: Contribution to journal › Article › peer-review

Abstract

Neighbouring cells can recognise and communicate with each other by direct binding between cell surface receptor and ligand pairs. Examples of cellular recognition events include pathogen entry into a host cell, sperm-egg fusion, and self/nonself discrimination by the immune system. Despite growing appreciation of cell surface recognition molecules as potential therapeutic targets, identifying key factors contributing to cellular recognition remains technically challenging to perform on a genome-wide scale. Recently, genome-scale clustered regularly interspaced short palindromic repeats (CRISPR) knockout or activation (CRISPR-KO/CRISPRa) screens have been applied to identify the molecular determinants of cellular recognition. In this review, we discuss how CRISPR-KO/CRISPRa screening has contributed to our understanding of cellular recognition processes, and how it can be applied to investigate these important interactions in a range of biological contexts. Copyright © 2020 The Authors. Published by Elsevier Ltd.. All rights reserved.

Original language	English
Pages (from-to)	619-627
Number of pages	9
Journal	TRENDS IN CELL BIOLOGY
Volume	30
Issue number	8
Early online date	25 Jun 2020
DOIs	https://doi.org/10.1016/j.tcb.2020.05.005
Publication status	Published - 1 Aug 2020

Bibliographical note

Keywords

cellular genetics
staffpaper

Access to Document

10.1016/j.tcb.2020.05.005

PIIS0962892420301021
© 2020 The Authors.
Final published version, 1.48 MBLicence: CC BY-NC-ND

https://linkinghub.elsevier.com/retrieve/pii/S0962892420301021

Cite this

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title = "Investigating cellular recognition using crispr/cas9 genetic screening.",

abstract = "Neighbouring cells can recognise and communicate with each other by direct binding between cell surface receptor and ligand pairs. Examples of cellular recognition events include pathogen entry into a host cell, sperm-egg fusion, and self/nonself discrimination by the immune system. Despite growing appreciation of cell surface recognition molecules as potential therapeutic targets, identifying key factors contributing to cellular recognition remains technically challenging to perform on a genome-wide scale. Recently, genome-scale clustered regularly interspaced short palindromic repeats (CRISPR) knockout or activation (CRISPR-KO/CRISPRa) screens have been applied to identify the molecular determinants of cellular recognition. In this review, we discuss how CRISPR-KO/CRISPRa screening has contributed to our understanding of cellular recognition processes, and how it can be applied to investigate these important interactions in a range of biological contexts. Copyright {\textcopyright} 2020 The Authors. Published by Elsevier Ltd.. All rights reserved.",

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