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Investigating cellular recognition using crispr/cas9 genetic screening.

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JournalTRENDS IN CELL BIOLOGY
DateE-pub ahead of print - 25 Jun 2020
DatePublished (current) - 1 Aug 2020
Issue number8
Volume30
Number of pages9
Pages (from-to)619-627
Early online date25/06/20
Original languageEnglish

Abstract

Neighbouring cells can recognise and communicate with each other by direct binding between cell surface receptor and ligand pairs. Examples of cellular recognition events include pathogen entry into a host cell, sperm-egg fusion, and self/nonself discrimination by the immune system. Despite growing appreciation of cell surface recognition molecules as potential therapeutic targets, identifying key factors contributing to cellular recognition remains technically challenging to perform on a genome-wide scale. Recently, genome-scale clustered regularly interspaced short palindromic repeats (CRISPR) knockout or activation (CRISPR-KO/CRISPRa) screens have been applied to identify the molecular determinants of cellular recognition. In this review, we discuss how CRISPR-KO/CRISPRa screening has contributed to our understanding of cellular recognition processes, and how it can be applied to investigate these important interactions in a range of biological contexts. Copyright © 2020 The Authors. Published by Elsevier Ltd.. All rights reserved.

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© 2020 The Authors.

    Research areas

  • cellular genetics, staffpaper

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