Investigating templating within Polymer-Scaffolded Dynamic Combinatorial Libraries

TY - JOUR

T1 - Investigating templating within Polymer-Scaffolded Dynamic Combinatorial Libraries

AU - Mahon, Clare S.

AU - Jackson, Alexander W.

AU - Murray, Benjamin S.

AU - A. Fulton, David

PY - 2013/1/21

Y1 - 2013/1/21

N2 - Polymer-Scaffolded Dynamic Combinatorial Libraries (PS-DCLs) have been prepared in aqueous solution by the reversible conjugation of two different acylhydrazide residues onto aldehyde-functionalized dimethylacrylamide-based polymer scaffolds. PS-DCLs have been shown by 1H NMR spectroscopic analysis to adapt their composition in response to addition of three macromolecular template species, namely poly(sodium-4-styrenesulfonate), bovine serum albumin and bovine trypsin. Compositional changes within PS-DCLs in response to template addition may be rationalized in terms of multivalent interactions between members of the PS-DCL and templates. A systematic evaluation of how properties of the polymer scaffold, including molecular weight and density of aldehyde functionalities, affect the behaviour of resultant PS-DCLs is detailed. Increasing the molecular weight of the polymer scaffold has been shown to induce a linear decrease in the amplification of the preferred residue upon template addition. A linear relationship between the molar weight percentage of the aldehyde functionalized monomer and the extent of amplification of the preferred residue upon template addition has also been demonstrated.

AB - Polymer-Scaffolded Dynamic Combinatorial Libraries (PS-DCLs) have been prepared in aqueous solution by the reversible conjugation of two different acylhydrazide residues onto aldehyde-functionalized dimethylacrylamide-based polymer scaffolds. PS-DCLs have been shown by 1H NMR spectroscopic analysis to adapt their composition in response to addition of three macromolecular template species, namely poly(sodium-4-styrenesulfonate), bovine serum albumin and bovine trypsin. Compositional changes within PS-DCLs in response to template addition may be rationalized in terms of multivalent interactions between members of the PS-DCL and templates. A systematic evaluation of how properties of the polymer scaffold, including molecular weight and density of aldehyde functionalities, affect the behaviour of resultant PS-DCLs is detailed. Increasing the molecular weight of the polymer scaffold has been shown to induce a linear decrease in the amplification of the preferred residue upon template addition. A linear relationship between the molar weight percentage of the aldehyde functionalized monomer and the extent of amplification of the preferred residue upon template addition has also been demonstrated.

UR - http://www.scopus.com/inward/record.url?scp=84870939986&partnerID=8YFLogxK

U2 - 10.1039/c2py20600e

DO - 10.1039/c2py20600e

M3 - Article

AN - SCOPUS:84870939986

VL - 4

SP - 368

EP - 377

JO - Polymer Chemistry

JF - Polymer Chemistry

SN - 1759-9954

IS - 2

ER -