IPIP27 coordinates PtdIns(4,5)P2 homeostasis for successful cytokinesis

TY - JOUR

T1 - IPIP27 coordinates PtdIns(4,5)P2 homeostasis for successful cytokinesis

AU - Carim, Sabrya C.

AU - Ben El Kadhi, Khaled

AU - Yan, Guanhua

AU - Sweeney, Sean

AU - Hickson, Gilles R.

AU - Carreno, Sebastien

AU - Lowe, Martin

N1 - Copyright © 2019 The Author(s). Published by Elsevier Ltd.. All rights reserved.

PY - 2019/3/4

Y1 - 2019/3/4

N2 - During cytokinesis, an actomyosin contractile ring drives the separation of the two daughter cells. A key molecule in this process is the inositol lipid PtdIns(4,5)P 2 , which recruits numerous factors to the equatorial region for contractile ring assembly. Despite the importance of PtdIns(4,5)P 2 in cytokinesis, the regulation of this lipid in cell division remains poorly understood. Here, we identify a role for IPIP27 in mediating cellular PtdIns(4,5)P 2 homeostasis. IPIP27 scaffolds the inositol phosphatase oculocerebrorenal syndrome of Lowe (OCRL) by coupling it to endocytic BAR domain proteins. Loss of IPIP27 causes accumulation of PtdIns(4,5)P 2 on aberrant endomembrane vacuoles, mislocalization of the cytokinetic machinery, and extensive cortical membrane blebbing. This phenotype is observed in Drosophila and human cells and can result in cytokinesis failure. We have therefore identified IPIP27 as a key modulator of cellular PtdIns(4,5)P 2 homeostasis required for normal cytokinesis. The results indicate that scaffolding of inositol phosphatase activity is critical for maintaining PtdIns(4,5)P 2 homeostasis and highlight a critical role for this process in cell division. Carim et al. reveal that the IPIP27 protein, which physically couples the inositol phosphatase OCRL to endocytic BAR domain proteins, is required for cellular phosphoinositide homeostasis and normal cell division. The results show the importance of physically scaffolding inositol phosphatase activity within cells.

AB - During cytokinesis, an actomyosin contractile ring drives the separation of the two daughter cells. A key molecule in this process is the inositol lipid PtdIns(4,5)P 2 , which recruits numerous factors to the equatorial region for contractile ring assembly. Despite the importance of PtdIns(4,5)P 2 in cytokinesis, the regulation of this lipid in cell division remains poorly understood. Here, we identify a role for IPIP27 in mediating cellular PtdIns(4,5)P 2 homeostasis. IPIP27 scaffolds the inositol phosphatase oculocerebrorenal syndrome of Lowe (OCRL) by coupling it to endocytic BAR domain proteins. Loss of IPIP27 causes accumulation of PtdIns(4,5)P 2 on aberrant endomembrane vacuoles, mislocalization of the cytokinetic machinery, and extensive cortical membrane blebbing. This phenotype is observed in Drosophila and human cells and can result in cytokinesis failure. We have therefore identified IPIP27 as a key modulator of cellular PtdIns(4,5)P 2 homeostasis required for normal cytokinesis. The results indicate that scaffolding of inositol phosphatase activity is critical for maintaining PtdIns(4,5)P 2 homeostasis and highlight a critical role for this process in cell division. Carim et al. reveal that the IPIP27 protein, which physically couples the inositol phosphatase OCRL to endocytic BAR domain proteins, is required for cellular phosphoinositide homeostasis and normal cell division. The results show the importance of physically scaffolding inositol phosphatase activity within cells.

KW - BAR domain protein

KW - IPIP27

KW - OCRL

KW - PtdIns(4,5)P

KW - SH3PX1

KW - actin

KW - cytokinesis

KW - endosome

KW - phosphoinositide

UR - http://www.scopus.com/inward/record.url?scp=85062156589&partnerID=8YFLogxK

U2 - 10.1016/j.cub.2019.01.043

DO - 10.1016/j.cub.2019.01.043

M3 - Article

C2 - 30799246

SN - 0960-9822

VL - 29

SP - 775

EP - 789

JO - Current Biology

JF - Current Biology

IS - 5

ER -