Rhizobium anhuiense sp. nov., isolated from effective nodules of Vicia faba and Pisum sativum grown in Southern China

TY - JOUR

T1 - Rhizobium anhuiense sp. nov., isolated from effective nodules of Vicia faba and Pisum sativum grown in Southern China

AU - Zhang, Yu Jing

AU - Zheng, Wen Tao

AU - Everall, Isobel

AU - Young, J Peter W

AU - Zhang, Xiao Xia

AU - Tian, Chang Fu

AU - Sui, Xin Hua

AU - Wang, En Tao

AU - Chen, Wen Xin

PY - 2015

Y1 - 2015

N2 - Four rhizobia like strains, isolated from root nodules of Pisum sativum and Vicia faba grown in Anhui and Jiangxi Provinces of China, were grouped into the genus Rhizobium but were distinct from all recognized Rhizobium species by phylogenetic analysis of 16S rRNA and housekeeping genes. The combined sequence of housekeeping genes atpD, recA and glnII for strains CCBAU 23252T was 86.9% to 95% similar to those of known Rhizobium species. All the four strains had nodC and nifH genes and could form effective nodules with Pisum sativum and Vicia faba, and ineffective nodules with Phaseolus vulgaris, but did not nodulate Glycine max, Arachis hypogaea, Medicago sativa, Trifolium repens or Lablab purpureus in cross-nodulation tests. Fatty acid composition, DNA-DNA relatedness and a series of phenotypic tests also separated these strains from the closely related species. Based on all the evidence, we propose a novel species Rhizobium anhuiense sp. nov., and designate CCBAU 23252T (=CGMCC 1.12621T =LMG 27729T) as the type strain. This strain was isolated from a root nodule of Vicia faba and has a GC content of 61.1 mol% (Tm).

AB - Four rhizobia like strains, isolated from root nodules of Pisum sativum and Vicia faba grown in Anhui and Jiangxi Provinces of China, were grouped into the genus Rhizobium but were distinct from all recognized Rhizobium species by phylogenetic analysis of 16S rRNA and housekeeping genes. The combined sequence of housekeeping genes atpD, recA and glnII for strains CCBAU 23252T was 86.9% to 95% similar to those of known Rhizobium species. All the four strains had nodC and nifH genes and could form effective nodules with Pisum sativum and Vicia faba, and ineffective nodules with Phaseolus vulgaris, but did not nodulate Glycine max, Arachis hypogaea, Medicago sativa, Trifolium repens or Lablab purpureus in cross-nodulation tests. Fatty acid composition, DNA-DNA relatedness and a series of phenotypic tests also separated these strains from the closely related species. Based on all the evidence, we propose a novel species Rhizobium anhuiense sp. nov., and designate CCBAU 23252T (=CGMCC 1.12621T =LMG 27729T) as the type strain. This strain was isolated from a root nodule of Vicia faba and has a GC content of 61.1 mol% (Tm).

U2 - 10.1099/ijs.0.000365

DO - 10.1099/ijs.0.000365

M3 - Article

C2 - 26025940

SN - 1466-5026

JO - International Journal of Systematic and Evolutionary Microbiology

JF - International Journal of Systematic and Evolutionary Microbiology

ER -