Isolation and Assessment of Single Long-Term Reconstituting Hematopoietic Stem Cells from Adult Mouse Bone Marrow.

DG Kent; BJ Dykstra; CJ Eaves

doi:10.1002/cpsc.10

Isolation and Assessment of Single Long-Term Reconstituting Hematopoietic Stem Cells from Adult Mouse Bone Marrow.

DG Kent, BJ Dykstra, CJ Eaves

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Hematopoietic stem cells with long-term repopulating activity can now be routinely obtained at purities of 40% to 50% from suspensions of adult mouse bone marrow. Here we describe robust protocols for both their isolation as CD45(+) EPCR(+) CD150(+) CD48(-) (ESLAM) cells using multiparameter cell sorting and for tracking their clonal growth and differentiation activity in irradiated mice transplanted with single ESLAM cells. The simplicity of these procedures makes them attractive for characterizing the molecular and biological properties of individual hematopoietic stem cells with unprecedented power and precision. © 2016 by John Wiley Sons, Inc.

Original language	English
Pages (from-to)	2A.4.1-2A.4.24
Journal	Current Protocols in Stem Cell Biology
Volume	38
Issue number	1
DOIs	https://doi.org/10.1002/cpsc.10
Publication status	Published - 17 Aug 2016

Access to Document

10.1002/cpsc.10

https://www.ncbi.nlm.nih.gov/pubmed/27532815

Cite this

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title = "Isolation and Assessment of Single Long-Term Reconstituting Hematopoietic Stem Cells from Adult Mouse Bone Marrow.",

abstract = "Hematopoietic stem cells with long-term repopulating activity can now be routinely obtained at purities of 40% to 50% from suspensions of adult mouse bone marrow. Here we describe robust protocols for both their isolation as CD45(+) EPCR(+) CD150(+) CD48(-) (ESLAM) cells using multiparameter cell sorting and for tracking their clonal growth and differentiation activity in irradiated mice transplanted with single ESLAM cells. The simplicity of these procedures makes them attractive for characterizing the molecular and biological properties of individual hematopoietic stem cells with unprecedented power and precision. {\textcopyright} 2016 by John Wiley Sons, Inc.",

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