Isolation and characterization of an amiloride-resistant mutant of Methanothermobacter thermautotrophicus possessing a defective Na+/H+ antiport

Stanislav Surin; L'ubomira Cubonova; Alan I. Majernik; Paul McDermott; James P. J. Chong; Peter Smigan

doi:10.1111/j.1574-6968.2007.00655.x

Isolation and characterization of an amiloride-resistant mutant of Methanothermobacter thermautotrophicus possessing a defective Na+/H+ antiport

Stanislav Surin, L'ubomira Cubonova, Alan I. Majernik, Paul McDermott, James P. J. Chong, Peter Smigan

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

A spontaneous mutant of Methanothermobacter thermautotrophicus resistant to the Na+/H+ antiporter inhibitor amiloride was isolated. The Na+/H+ exchanger activity in the mutant cells was remarkably decreased in comparison with wild-type cells. Methanogenesis rates in the mutant strain were higher than wild-type cells and resistant to the inhibitory effect of 2 mM amiloride. In contrast, methanogenesis in wild-type cells was completely inhibited by the same amiloride concentration. ATP synthesis driven by methanogenic electron transport or by an electrogenic potassium efflux in the presence of sodium ions was significantly enhanced in the mutant cells. ATP synthesis driven by potassium diffusion potential was profoundly inhibited in wild-type cells by the presence of uncoupler 3,3',4',5- tetrachlorosalicylanilide and sodium ions, whereas c. 50% inhibition was observed in the mutant cells under the same conditions.

Original language	English
Pages (from-to)	301-308
Number of pages	8
Journal	FEMS microbiology letters
Volume	269
Issue number	2
DOIs	https://doi.org/10.1111/j.1574-6968.2007.00655.x
Publication status	Published - Apr 2007

Keywords

sodium
proton exchange
ATP synthesis
archaea
methanogen
METHANOBACTERIUM-THERMOAUTOTROPHICUM
HETERODISULFIDE REDUCTASE
METHANOGENIC ARCHAEA
TRANSLOCATING METHYLTRANSFERASE
ELECTRON-TRANSPORT
ATP SYNTHESIS
SODIUM
MEMBRANE
DRIVEN
PROTEINS

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10.1111/j.1574-6968.2007.00655.x

Cite this

@article{5a8548b5b146483ab10c079f6b09ddcc,

title = "Isolation and characterization of an amiloride-resistant mutant of Methanothermobacter thermautotrophicus possessing a defective Na+/H+ antiport",

abstract = "A spontaneous mutant of Methanothermobacter thermautotrophicus resistant to the Na+/H+ antiporter inhibitor amiloride was isolated. The Na+/H+ exchanger activity in the mutant cells was remarkably decreased in comparison with wild-type cells. Methanogenesis rates in the mutant strain were higher than wild-type cells and resistant to the inhibitory effect of 2 mM amiloride. In contrast, methanogenesis in wild-type cells was completely inhibited by the same amiloride concentration. ATP synthesis driven by methanogenic electron transport or by an electrogenic potassium efflux in the presence of sodium ions was significantly enhanced in the mutant cells. ATP synthesis driven by potassium diffusion potential was profoundly inhibited in wild-type cells by the presence of uncoupler 3,3',4',5- tetrachlorosalicylanilide and sodium ions, whereas c. 50% inhibition was observed in the mutant cells under the same conditions.",

keywords = "sodium, proton exchange, ATP synthesis, archaea, methanogen, METHANOBACTERIUM-THERMOAUTOTROPHICUM, HETERODISULFIDE REDUCTASE, METHANOGENIC ARCHAEA, TRANSLOCATING METHYLTRANSFERASE, ELECTRON-TRANSPORT, ATP SYNTHESIS, SODIUM, MEMBRANE, DRIVEN, PROTEINS",

author = "Stanislav Surin and L'ubomira Cubonova and Majernik, {Alan I.} and Paul McDermott and Chong, {James P. J.} and Peter Smigan",

year = "2007",

month = apr,

doi = "10.1111/j.1574-6968.2007.00655.x",

language = "English",

volume = "269",

pages = "301--308",

journal = "FEMS microbiology letters",

issn = "0378-1097",

publisher = "Wiley-Blackwell",

number = "2",

}

TY - JOUR

T1 - Isolation and characterization of an amiloride-resistant mutant of Methanothermobacter thermautotrophicus possessing a defective Na+/H+ antiport

AU - Surin, Stanislav

AU - Cubonova, L'ubomira

AU - Majernik, Alan I.

AU - McDermott, Paul

AU - Chong, James P. J.

AU - Smigan, Peter

PY - 2007/4

Y1 - 2007/4

N2 - A spontaneous mutant of Methanothermobacter thermautotrophicus resistant to the Na+/H+ antiporter inhibitor amiloride was isolated. The Na+/H+ exchanger activity in the mutant cells was remarkably decreased in comparison with wild-type cells. Methanogenesis rates in the mutant strain were higher than wild-type cells and resistant to the inhibitory effect of 2 mM amiloride. In contrast, methanogenesis in wild-type cells was completely inhibited by the same amiloride concentration. ATP synthesis driven by methanogenic electron transport or by an electrogenic potassium efflux in the presence of sodium ions was significantly enhanced in the mutant cells. ATP synthesis driven by potassium diffusion potential was profoundly inhibited in wild-type cells by the presence of uncoupler 3,3',4',5- tetrachlorosalicylanilide and sodium ions, whereas c. 50% inhibition was observed in the mutant cells under the same conditions.

AB - A spontaneous mutant of Methanothermobacter thermautotrophicus resistant to the Na+/H+ antiporter inhibitor amiloride was isolated. The Na+/H+ exchanger activity in the mutant cells was remarkably decreased in comparison with wild-type cells. Methanogenesis rates in the mutant strain were higher than wild-type cells and resistant to the inhibitory effect of 2 mM amiloride. In contrast, methanogenesis in wild-type cells was completely inhibited by the same amiloride concentration. ATP synthesis driven by methanogenic electron transport or by an electrogenic potassium efflux in the presence of sodium ions was significantly enhanced in the mutant cells. ATP synthesis driven by potassium diffusion potential was profoundly inhibited in wild-type cells by the presence of uncoupler 3,3',4',5- tetrachlorosalicylanilide and sodium ions, whereas c. 50% inhibition was observed in the mutant cells under the same conditions.

KW - sodium

KW - proton exchange

KW - ATP synthesis

KW - archaea

KW - methanogen

KW - METHANOBACTERIUM-THERMOAUTOTROPHICUM

KW - HETERODISULFIDE REDUCTASE

KW - METHANOGENIC ARCHAEA

KW - TRANSLOCATING METHYLTRANSFERASE

KW - ELECTRON-TRANSPORT

KW - ATP SYNTHESIS

KW - SODIUM

KW - MEMBRANE

KW - DRIVEN

KW - PROTEINS

U2 - 10.1111/j.1574-6968.2007.00655.x

DO - 10.1111/j.1574-6968.2007.00655.x

M3 - Article

SN - 0378-1097

VL - 269

SP - 301

EP - 308

JO - FEMS microbiology letters

JF - FEMS microbiology letters

IS - 2

ER -