Two novel cDNAs, Plubc1 and Plubc2 encoding ubiquitin-conjugating enzyme E2, were isolated from a Pavlova lutheri cDNA library. They are each encoded by single copy genes in the algae genome. Sequence comparison with plant, yeast and algal E2 sequences showed that PlUBC1 and PlUBC2 are members of new E2 subfamilies. Time-course expression analysis of the two cDNAs revealed that Plubc1 is transitionally over-expressed at the end of the exponential phase of growth of the culture, while Plubc2 is constitutively expressed at the same level throughout the cell growth. The phylogenetic study and the different expression patterns suggest that these two enzymes could exhibit different physiological functions in P. lutheri. The partial sequence of the 18S rRNA gene and the full-length cDNA sequence of Plubc1 and Plubc2 reported in this paper will appear in the Genbank database under the accession numbers AY135218, AY135219 and AY135220 respectively.
|Number of pages||8|
|Journal||Journal of applied phycology|
|Publication status||Published - Sept 2003|
- E2 ubiquitin-conjugating enzyme
- growth stages
- Pavlova lutheri
- CHROMOPHYTIC ALGA