By the same authors

From the same journal

Isolation of two cDNAs encoding E2 ubiquitin-conjugating enzymes from Pavlova lutheri (Haptophyceae).

Research output: Contribution to journalArticle

Author(s)

Department/unit(s)

Publication details

JournalJournal of applied phycology
DatePublished - Sep 2003
Issue number5
Volume15
Number of pages8
Pages (from-to)425-432
Original languageEnglish

Abstract

Two novel cDNAs, Plubc1 and Plubc2 encoding ubiquitin-conjugating enzyme E2, were isolated from a Pavlova lutheri cDNA library. They are each encoded by single copy genes in the algae genome. Sequence comparison with plant, yeast and algal E2 sequences showed that PlUBC1 and PlUBC2 are members of new E2 subfamilies. Time-course expression analysis of the two cDNAs revealed that Plubc1 is transitionally over-expressed at the end of the exponential phase of growth of the culture, while Plubc2 is constitutively expressed at the same level throughout the cell growth. The phylogenetic study and the different expression patterns suggest that these two enzymes could exhibit different physiological functions in P. lutheri. The partial sequence of the 18S rRNA gene and the full-length cDNA sequence of Plubc1 and Plubc2 reported in this paper will appear in the Genbank database under the accession numbers AY135218, AY135219 and AY135220 respectively.

    Research areas

  • E2 ubiquitin-conjugating enzyme, growth stages, Pavlova lutheri, CHROMOPHYTIC ALGA, PROTEIN, MICROALGAE, IDENTIFICATION, PLASTIDS, CLONING, HOMOLOG, GENOME, ORIGIN, PLANTS

Discover related content

Find related publications, people, projects, datasets and more using interactive charts.

View graph of relations