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klf2ash317 Mutant Zebrafish Do Not Recapitulate Morpholino-Induced Vascular and Haematopoietic Phenotypes

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Published copy (DOI)

Author(s)

  • Peter Novodvorsky
  • Oliver Watson
  • Caroline Gray
  • Robert N Wilkinson
  • Scott Reeve
  • Carl Smythe
  • Richard Beniston
  • Karen Plant
  • Richard Maguire
  • Alexander M K Rothman
  • Stone Elworthy
  • Fredericus J M van Eeden
  • Timothy J A Chico

Department/unit(s)

Publication details

JournalPLoS ONE
DatePublished - 2015
Issue number10
Volume10
Pages (from-to)e0141611
Original languageEnglish

Abstract

INTRODUCTION AND OBJECTIVES: The zinc-finger transcription factor Krϋppel-like factor 2 (KLF2) transduces blood flow into molecular signals responsible for a wide range of responses within the vasculature. KLF2 maintains a healthy, quiescent endothelial phenotype. Previous studies report a range of phenotypes following morpholino antisense oligonucleotide-induced klf2a knockdown in zebrafish. Targeted genome editing is an increasingly applied method for functional assessment of candidate genes. We therefore generated a stable klf2a mutant zebrafish and characterised its cardiovascular and haematopoietic development.

METHODS AND RESULTS: Using Transcription Activator-Like Effector Nucleases (TALEN) we generated a klf2a mutant (klf2ash317) with a 14bp deletion leading to a premature stop codon in exon 2. Western blotting confirmed loss of wild type Klf2a protein and the presence of a truncated protein in klf2ash317 mutants. Homozygous klf2ash317 mutants exhibit no defects in vascular patterning, survive to adulthood and are fertile, without displaying previously described morphant phenotypes such as high-output cardiac failure, reduced haematopoetic stem cell (HSC) development or impaired formation of the 5th accessory aortic arch. Homozygous klf2ash317 mutation did not reduce angiogenesis in zebrafish with homozygous mutations in von Hippel Lindau (vhl), a form of angiogenesis that is dependent on blood flow. We examined expression of three klf family members in wildtype and klf2ash317 zebrafish. We detected vascular expression of klf2b (but not klf4a or biklf/klf4b/klf17) in wildtypes but found no differences in expression that might account for the lack of phenotype in klf2ash317 mutants. klf2b morpholino knockdown did not affect heart rate or impair formation of the 5th accessory aortic arch in either wildtypes or klf2ash317 mutants.

CONCLUSIONS: The klf2ash317 mutation produces a truncated Klf2a protein but, unlike morpholino induced klf2a knockdown, does not affect cardiovascular development.

    Research areas

  • Animals, Cardiovascular System, Gene Expression Regulation, Developmental, Genotype, Hematopoietic System, Humans, Kruppel-Like Transcription Factors, Morphogenesis, Morpholinos, Mutation, Signal Transduction, Zebrafish, Zebrafish Proteins, Journal Article, Research Support, Non-U.S. Gov't

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