TY - JOUR
T1 - Leishmania genome dynamics during environmental adaptation reveals strain-specific differences in gene copy number variation, karyotype instability, and telomeric amplification
AU - Bussotti, Giovanni
AU - Gouzelou, Evi
AU - Cortes Boite, Mariana
AU - Kherachi, Ihcen
AU - Harrat, Zoubir
AU - Eddaikra, Naouel
AU - Mottram, Jeremy Charles
AU - Antoniou, Maria
AU - Christodoulou, Vasiliki
AU - Bali, Aymen
AU - Guerfali, Fatma Z.
AU - Laouini, Dhafer
AU - Mukhtar, Maowla
AU - Dumetz, Franck
AU - Dujardin, Jean-Claude
AU - Smirlis, Despina
AU - Lechat, Pierre
AU - Pescher, Pascale
AU - El Hamouchi, Adil
AU - Lemrani, Meryem
AU - Chicharro, Carmen
AU - Llanes-Acevedo, Ivonne Pamela
AU - Botana, Laura
AU - Cruz, Israel
AU - Moreno, Javier
AU - Jeddi, Fakhri
AU - Aoun, Karim
AU - Bouratbine, Aida
AU - Cupolillo, Elisa
AU - Spath, Gerald F.
N1 - © 2018 Bussotti et al.
PY - 2018/11/6
Y1 - 2018/11/6
N2 - Protozoan parasites of the genus Leishmania adapt to environmental change through chromosome and gene copy number variations. Only little is known on external or intrinsic factors that govern Leishmania genomic adaptation. Here, by conducting longitudinal genome analyses of ten new Leishmania clinical isolates, we uncovered important differences in gene copy number among genetically highly related strains and revealed gain and loss of gene copies as potential drivers of long-term environmental adaptation in the field. In contrast, chromosome rather than gene amplification was associated with short-term environmental adaptation to in vitro culture. Karyotypic solutions were highly reproducible but unique for a given strain, suggesting that chromosome amplification is under positive selection and dependent on species- and strain-specific, intrinsic factors. We revealed a progressive increase in read depth towards the chromosome ends for various Leishmania isolates, which may represent a non-classical mechanism of telomere maintenance that can preserve integrity of chromosome ends during selection for fast in vitro growth. Together our data draw a complex picture of Leishmania genomic adaptation in the field and in culture, which is driven by a combination of intrinsic genetic factors that generate strain-specific, phenotypic variations, which are under environmental selection and allow for fitness gain.
AB - Protozoan parasites of the genus Leishmania adapt to environmental change through chromosome and gene copy number variations. Only little is known on external or intrinsic factors that govern Leishmania genomic adaptation. Here, by conducting longitudinal genome analyses of ten new Leishmania clinical isolates, we uncovered important differences in gene copy number among genetically highly related strains and revealed gain and loss of gene copies as potential drivers of long-term environmental adaptation in the field. In contrast, chromosome rather than gene amplification was associated with short-term environmental adaptation to in vitro culture. Karyotypic solutions were highly reproducible but unique for a given strain, suggesting that chromosome amplification is under positive selection and dependent on species- and strain-specific, intrinsic factors. We revealed a progressive increase in read depth towards the chromosome ends for various Leishmania isolates, which may represent a non-classical mechanism of telomere maintenance that can preserve integrity of chromosome ends during selection for fast in vitro growth. Together our data draw a complex picture of Leishmania genomic adaptation in the field and in culture, which is driven by a combination of intrinsic genetic factors that generate strain-specific, phenotypic variations, which are under environmental selection and allow for fitness gain.
U2 - 10.1128/mBio.01399-18
DO - 10.1128/mBio.01399-18
M3 - Article
SN - 2161-2129
VL - 9
JO - MBio
JF - MBio
IS - 6
M1 - e01399-18
ER -