Leishmania major: clathrin and adaptin complexes of an intra-cellular parasite

Paul W Denny, Gareth W Morgan, Mark C Field, Deborah F Smith

Research output: Contribution to journalArticlepeer-review


To investigate the role of clathrin-mediated trafficking during the Leishmania lifecycle, open reading frames encoding clathrin heavy chain and the beta-adaptins, major components of the adaptor complexes, have been analysed both in silico and experimentally. The Leishmania genome encodes three beta-adaptins, which arose at a time predating speciation of these divergent trypanosomatids. Unlike Trypanosoma brucei, both clathrin heavy chain and beta-adaptin1 are constitutively expressed throughout the Leishmania life cycle. Clathrin relocalises in amastigotes relative to promastigotes, consistent with developmental alterations to the morphology of the endo-membrane system.

Original languageEnglish
Pages (from-to)33-7
Number of pages5
JournalExperimental parasitology
Issue number1
Publication statusPublished - Jan 2005


  • Adaptor Protein Complex beta Subunits
  • Animals
  • Antibodies, Protozoan
  • Blotting, Western
  • Clathrin Heavy Chains
  • Cross Reactions
  • Fluorescent Antibody Technique
  • Gene Expression Regulation, Developmental
  • Genome, Protozoan
  • Golgi Apparatus
  • Leishmania major
  • Mice
  • Mice, Inbred BALB C
  • Open Reading Frames
  • Phylogeny

Cite this