Abstract
To investigate the role of clathrin-mediated trafficking during the Leishmania lifecycle, open reading frames encoding clathrin heavy chain and the beta-adaptins, major components of the adaptor complexes, have been analysed both in silico and experimentally. The Leishmania genome encodes three beta-adaptins, which arose at a time predating speciation of these divergent trypanosomatids. Unlike Trypanosoma brucei, both clathrin heavy chain and beta-adaptin1 are constitutively expressed throughout the Leishmania life cycle. Clathrin relocalises in amastigotes relative to promastigotes, consistent with developmental alterations to the morphology of the endo-membrane system.
Original language | English |
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Pages (from-to) | 33-7 |
Number of pages | 5 |
Journal | Experimental parasitology |
Volume | 109 |
Issue number | 1 |
DOIs | |
Publication status | Published - Jan 2005 |
Keywords
- Adaptor Protein Complex beta Subunits
- Animals
- Antibodies, Protozoan
- Blotting, Western
- Clathrin Heavy Chains
- Cross Reactions
- Fluorescent Antibody Technique
- Gene Expression Regulation, Developmental
- Genome, Protozoan
- Golgi Apparatus
- Leishmania major
- Mice
- Mice, Inbred BALB C
- Open Reading Frames
- Phylogeny