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Localization of chitinolytic activities in Fagus sylvatica mycorrhizas

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Author(s)

  • A Hodge
  • I J Alexander
  • G W Gooday
  • F A Williamson

Department/unit(s)

Publication details

JournalMYCORRHIZA
DatePublished - May 1996
Issue number3
Volume6
Number of pages7
Pages (from-to)181-187
Original languageEnglish

Abstract

Localization of chitinolytic activities in Fagus sylvatica (beech) mycorrhizas was examined using a range of fluorogenic 4-methylumbelliferyl [4-MU-(GlcNAc)(1-4)] substrates in order to distinguish between exochitinase, endochitinase and beta-N-acetylglucosaminidase activities. The validity of the technique was confirmed using onion epidermis cells. In the beech mycorrhiza, endochitinase activity was not detectable above background fluorescence. Exochitinase activity was detected in the fungal sheath and the Hartig net. beta-N-Acetylglucosaminidase activity was also mainly associated with the fungal sheath and Hartig net. Individual fungal hyphae extending from these structures also showed substantial beta-N-acetylglucosaminidase activity. The cortical cell walls of the host in the Hartig net region also fluoresced brightly. The localization of beta-N-acetylglucosaminidase activity was confirmed using a chromogenic histochemical reagent, 5-bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide (X-GlcNAc).

    Research areas

  • ectomycorrhizae, chitinolytic activities, localization, 4-methylumbelliferyl substrates, [4-MU-(GlcNAc)1-4], 5-bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide (X-GlcNAc), TRICHODERMA-HARZIANUM, ANTIFUNGAL ACTIVITY, PLANT CHITINASES, ROOTS, PURIFICATION, ENZYMES, ALLIUM, FUNGI, CELLS

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