Low-temperature plasma treatment induces DNA damage leading to necrotic cell death in primary prostate epithelial cells

TY - JOUR

T1 - Low-temperature plasma treatment induces DNA damage leading to necrotic cell death in primary prostate epithelial cells

AU - Hirst, A. M.

AU - Simms, M. S.

AU - Mann, V. M.

AU - Maitland, N. J.

AU - O'connell, D.

AU - Frame, F. M.

PY - 2015/4/28

Y1 - 2015/4/28

N2 - Background:In recent years, the rapidly advancing field of low-temperature atmospheric pressure plasmas has shown considerable promise for future translational biomedical applications, including cancer therapy, through the generation of reactive oxygen and nitrogen species.Method:The cytopathic effect of low-temperature plasma was first verified in two commonly used prostate cell lines: BPH-1 and PC-3 cells. The study was then extended to analyse the effects in paired normal and tumour (Gleason grade 7) prostate epithelial cells cultured directly from patient tissue. Hydrogen peroxide (H 2 O 2) and staurosporine were used as controls throughout.Results:Low-temperature plasma (LTP) exposure resulted in high levels of DNA damage, a reduction in cell viability, and colony-forming ability. H 2 O 2 formed in the culture medium was a likely facilitator of these effects. Necrosis and autophagy were recorded in primary cells, whereas cell lines exhibited apoptosis and necrosis.Conclusions:This study demonstrates that LTP treatment causes cytotoxic insult in primary prostate cells, leading to rapid necrotic cell death. It also highlights the need to study primary cultures in order to gain more realistic insight into patient response.

AB - Background:In recent years, the rapidly advancing field of low-temperature atmospheric pressure plasmas has shown considerable promise for future translational biomedical applications, including cancer therapy, through the generation of reactive oxygen and nitrogen species.Method:The cytopathic effect of low-temperature plasma was first verified in two commonly used prostate cell lines: BPH-1 and PC-3 cells. The study was then extended to analyse the effects in paired normal and tumour (Gleason grade 7) prostate epithelial cells cultured directly from patient tissue. Hydrogen peroxide (H 2 O 2) and staurosporine were used as controls throughout.Results:Low-temperature plasma (LTP) exposure resulted in high levels of DNA damage, a reduction in cell viability, and colony-forming ability. H 2 O 2 formed in the culture medium was a likely facilitator of these effects. Necrosis and autophagy were recorded in primary cells, whereas cell lines exhibited apoptosis and necrosis.Conclusions:This study demonstrates that LTP treatment causes cytotoxic insult in primary prostate cells, leading to rapid necrotic cell death. It also highlights the need to study primary cultures in order to gain more realistic insight into patient response.

KW - low-temperature plasma

KW - necrosis

KW - primary epithelial cells

KW - prostate cancer

KW - reactive species

UR - http://www.scopus.com/inward/record.url?scp=84928769128&partnerID=8YFLogxK

U2 - 10.1038/bjc.2015.113

DO - 10.1038/bjc.2015.113

M3 - Article

AN - SCOPUS:84928769128

SN - 0007-0920

VL - 112

SP - 1536

EP - 1545

JO - British journal of cancer

JF - British journal of cancer

IS - 9

ER -