Magnetically-Triggered Release of Entrapped Bioactive Proteins from Thermally Responsive Polymer-Coated Iron Oxide Nanoparticles for Stem Cell Proliferation

Matthew Walker; Iain Gordon Will; Andrew Pratt; Victor Chechik; Paul Genever; Dani Ungar

doi:10.1021/acsanm.0c01167

Magnetically-Triggered Release of Entrapped Bioactive Proteins from Thermally Responsive Polymer-Coated Iron Oxide Nanoparticles for Stem Cell Proliferation

Matthew Walker, Iain Gordon Will, Andrew Pratt, Victor Chechik, Paul Genever, Dani Ungar

Research output: Contribution to journal › Letter › peer-review

Abstract

Nanoparticles could conceal bioactive proteins during therapeutic delivery, avoiding side effects. Superparamagnetic iron oxide nanoparticles (SPIONs) coated with a temperature-sensitive polymer were tested for protein release. We show that coated SPIONs can entrap test proteins and release them in a temperature-controlled manner in a biological system. Magnetically heating SPIONs triggered protein release at bulk solution temperatures below polymer transition. The entrapped growth factor Wnt3a was inactive until magnetically-triggered release, upon which it could increase mesenchymal stem cell proliferation. Once chemically adjusting polymer transition above body temperature this system could be used for targeted cell stimulation in model animals and humans.

Original language	English
Pages (from-to)	5008-5013
Number of pages	6
Journal	ACS Applied Nano Materials
Volume	3
Issue number	6
Early online date	15 May 2020
DOIs	https://doi.org/10.1021/acsanm.0c01167
Publication status	Published - 26 Jun 2020

Keywords

superparamagnetic iron oxide nanoparticles; mesenchymal stem cells; in vivo protein delivery; temperature sensitive polymer; magnetic protein release; polymer coated nanoparticles

Access to Document

10.1021/acsanm.0c01167Licence: CC BY

acsanm.0c01167
© 2020 American Chemical Society
Final published version, 2.78 MBLicence: CC BY

Victor Chechik
- victor.chechikyork.acuk
- Chemistry - Professor
Person: Academic

Magnetic nanoparticle delivery to MSCs, EPSRC £60K.
Ungar, D. (Principal investigator), Genever, P. (Principal investigator) & Chechik, V. (Principal investigator)
1/10/15 → 30/09/18
Project: Other project › Miscellaneous project

Cite this

@article{76bedd4a988642799deb8287e76c7692,

title = "Magnetically-Triggered Release of Entrapped Bioactive Proteins from Thermally Responsive Polymer-Coated Iron Oxide Nanoparticles for Stem Cell Proliferation",

abstract = "Nanoparticles could conceal bioactive proteins during therapeutic delivery, avoiding side effects. Superparamagnetic iron oxide nanoparticles (SPIONs) coated with a temperature-sensitive polymer were tested for protein release. We show that coated SPIONs can entrap test proteins and release them in a temperature-controlled manner in a biological system. Magnetically heating SPIONs triggered protein release at bulk solution temperatures below polymer transition. The entrapped growth factor Wnt3a was inactive until magnetically-triggered release, upon which it could increase mesenchymal stem cell proliferation. Once chemically adjusting polymer transition above body temperature this system could be used for targeted cell stimulation in model animals and humans.",

keywords = "superparamagnetic iron oxide nanoparticles; mesenchymal stem cells; in vivo protein delivery; temperature sensitive polymer; magnetic protein release; polymer coated nanoparticles",

author = "Matthew Walker and Will, {Iain Gordon} and Andrew Pratt and Victor Chechik and Paul Genever and Dani Ungar",

year = "2020",

month = jun,

day = "26",

doi = "10.1021/acsanm.0c01167",

language = "English",

volume = "3",

pages = "5008--5013",

journal = "ACS Applied Nano Materials",

issn = "2574-0970",

publisher = "American Chemical Society",

number = "6",

}

TY - JOUR

T1 - Magnetically-Triggered Release of Entrapped Bioactive Proteins from Thermally Responsive Polymer-Coated Iron Oxide Nanoparticles for Stem Cell Proliferation

AU - Walker, Matthew

AU - Will, Iain Gordon

AU - Pratt, Andrew

AU - Chechik, Victor

AU - Genever, Paul

AU - Ungar, Dani

PY - 2020/6/26

Y1 - 2020/6/26

N2 - Nanoparticles could conceal bioactive proteins during therapeutic delivery, avoiding side effects. Superparamagnetic iron oxide nanoparticles (SPIONs) coated with a temperature-sensitive polymer were tested for protein release. We show that coated SPIONs can entrap test proteins and release them in a temperature-controlled manner in a biological system. Magnetically heating SPIONs triggered protein release at bulk solution temperatures below polymer transition. The entrapped growth factor Wnt3a was inactive until magnetically-triggered release, upon which it could increase mesenchymal stem cell proliferation. Once chemically adjusting polymer transition above body temperature this system could be used for targeted cell stimulation in model animals and humans.

AB - Nanoparticles could conceal bioactive proteins during therapeutic delivery, avoiding side effects. Superparamagnetic iron oxide nanoparticles (SPIONs) coated with a temperature-sensitive polymer were tested for protein release. We show that coated SPIONs can entrap test proteins and release them in a temperature-controlled manner in a biological system. Magnetically heating SPIONs triggered protein release at bulk solution temperatures below polymer transition. The entrapped growth factor Wnt3a was inactive until magnetically-triggered release, upon which it could increase mesenchymal stem cell proliferation. Once chemically adjusting polymer transition above body temperature this system could be used for targeted cell stimulation in model animals and humans.

KW - superparamagnetic iron oxide nanoparticles; mesenchymal stem cells; in vivo protein delivery; temperature sensitive polymer; magnetic protein release; polymer coated nanoparticles

U2 - 10.1021/acsanm.0c01167

DO - 10.1021/acsanm.0c01167

M3 - Letter

SN - 2574-0970

VL - 3

SP - 5008

EP - 5013

JO - ACS Applied Nano Materials

JF - ACS Applied Nano Materials

IS - 6

ER -

Magnetically-Triggered Release of Entrapped Bioactive Proteins from Thermally Responsive Polymer-Coated Iron Oxide Nanoparticles for Stem Cell Proliferation

Abstract

Keywords

Access to Document

Profiles

Victor Chechik

Projects

Magnetic nanoparticle delivery to MSCs, EPSRC £60K.

Cite this