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Mallard Blue: A High-Affinity Selective Heparin Sensor That Operates in Highly Competitive Media

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JournalJournal of the American Chemical Society
DatePublished - 27 Feb 2013
Issue number8
Volume135
Pages (from-to)2911-2914
Original languageEnglish

Abstract

We report the simple synthesis and full investigation of a novel heparin binding dye, mallard blue, an arginine-functionalized thionine. This dye binds heparin in highly competitive media, including water with high levels of competitive electrolyte, buffered aqueous solution and human serum. The dye reports on heparin levels by a significant change in its UV-vis spectroscopic profile. Molecular dynamics modeling provides detailed insight into the binding mode. Heparin binding is shown to be selective over other glycosaminoglycans, such as hyaluronic acid and chondroitin sulfate. Importantly, we demonstrate that, in the most competitive conditions, mallard blue outperforms standard dyes used for heparin sensing such as azure A.

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