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Mannitol metabolism in brown algae involves a new phosphatase family

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Published copy (DOI)

Author(s)

  • Agnès Groisillier
  • Zhanru Shao
  • Gurvan Michel
  • Sophie Goulitquer
  • Patricia Bonin
  • Stefan Krahulec
  • Bernd Nidetzky
  • Delin Duan
  • Catherine Boyen
  • Thierry Tonon

Department/unit(s)

Publication details

JournalJournal of Experimental Botany
DateE-pub ahead of print - 9 Dec 2013
DatePublished (current) - 1 Feb 2014
Issue number2
Volume65
Number of pages12
Pages (from-to)559-570
Early online date9/12/13
Original languageEnglish

Abstract

Brown algae belong to a phylogenetic lineage distantly related to green plants and animals, and are found predominantly in the intertidal zone, a harsh and frequently changing environment. Because of their unique evolutionary history and of their habitat, brown algae feature several peculiarities in their metabolism. One of these is the mannitol cycle, which plays a central role in their physiology, as mannitol acts as carbon storage, osmoprotectant, and antioxidant. This polyol is derived directly from the photoassimilate fructose-6-phosphate via the action of a mannitol-1-phosphate dehydrogenase and a mannitol-1-phosphatase (M1Pase). Genome analysis of the brown algal model Ectocarpus siliculosus allowed identification of genes potentially involved in the mannitol cycle. Among these, two genes coding for haloacid dehalogenase (HAD)-like enzymes were suggested to correspond to M1Pase activity, and thus were named EsM1Pase1 and EsM1Pase2, respectively. To test this hypothesis, both genes were expressed in Escherichia coli. Recombinant EsM1Pase2 was shown to hydrolyse the phosphate group from mannitol-1-phosphate to produce mannitol but was not active on the hexose monophosphates tested. Gene expression analysis showed that transcription of both E. siliculosus genes was under the influence of the diurnal cycle. Sequence analysis and three-dimensional homology modelling indicated that EsM1Pases, and their orthologues in Prasinophytes, should be seen as founding members of a new family of phosphatase with original substrate specificity within the HAD superfamily of proteins. This is the first report describing the characterization of a gene encoding M1Pase activity in photosynthetic organisms.

    Research areas

  • Algal Proteins/chemistry, Amino Acid Sequence, Carbon/metabolism, Gene Expression Regulation, Plant, Light, Mannitol/metabolism, Models, Molecular, Molecular Sequence Data, Molecular Weight, Multigene Family, Phaeophyta/enzymology, Phosphoric Monoester Hydrolases/isolation & purification, Recombinant Proteins/metabolism, Sequence Alignment

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