Mapping-by-Sequencing Identifies HvPHYTOCHROME C as a Candidate Gene for the early maturity 5 Locus Modulating the Circadian Clock and Photoperiodic Flowering in Barley

Artem Pankin; Chiara Campoli; Xue Dong; Benjamin Kilian; Rajiv Sharma; Axel Himmelbach; Reena Saini; Seth J Davis; Nils Stein; Korbinian Schneeberger; Maria von Korff

doi:10.1534/genetics.114.165613

Mapping-by-Sequencing Identifies HvPHYTOCHROME C as a Candidate Gene for the early maturity 5 Locus Modulating the Circadian Clock and Photoperiodic Flowering in Barley

Artem Pankin, Chiara Campoli, Xue Dong, Benjamin Kilian, Rajiv Sharma, Axel Himmelbach, Reena Saini, Seth J Davis, Nils Stein, Korbinian Schneeberger, Maria von Korff

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Phytochromes play an important role in light signaling and photoperiodic control of flowering time in plants. Here we propose that the red/far-red light photoreceptor HvPHYTOCHROME C (HvPHYC), carrying a mutation in a conserved region of the GAF domain, is a candidate underlying the early maturity 5 locus in barley (Hordeum vulgare L.). We fine-mapped the gene using a mapping-by-sequencing approach applied on the whole-exome capture data from bulked early flowering segregants derived from a backcross of the Bowman(eam5) introgression line. We demonstrate that eam5 disrupts circadian expression of clock genes. Moreover, it interacts with the major photoperiod response gene Ppd-H1 to accelerate flowering under non-inductive short days. Our results suggest that HvPHYC participates in transmission of light signals to the circadian clock and thus modulates light-dependent processes such as photoperiodic regulation of flowering.

Original language	English
Pages (from-to)	383-396
Journal	Human Genetics
Volume	198
Issue number	1
Early online date	3 Jul 2014
DOIs	https://doi.org/10.1534/genetics.114.165613
Publication status	Published - 1 Sept 2014

Bibliographical note

Access to Document

10.1534/genetics.114.165613

Cite this

Pankin, A., Campoli, C., Dong, X., Kilian, B., Sharma, R., Himmelbach, A., Saini, R., Davis, S. J., Stein, N., Schneeberger, K., & von Korff, M. (2014). Mapping-by-Sequencing Identifies HvPHYTOCHROME C as a Candidate Gene for the early maturity 5 Locus Modulating the Circadian Clock and Photoperiodic Flowering in Barley. Human Genetics, 198(1), 383-396. https://doi.org/10.1534/genetics.114.165613

@article{488ca503760a47f6b2d2298099830bc6,

title = "Mapping-by-Sequencing Identifies HvPHYTOCHROME C as a Candidate Gene for the early maturity 5 Locus Modulating the Circadian Clock and Photoperiodic Flowering in Barley",

abstract = "Phytochromes play an important role in light signaling and photoperiodic control of flowering time in plants. Here we propose that the red/far-red light photoreceptor HvPHYTOCHROME C (HvPHYC), carrying a mutation in a conserved region of the GAF domain, is a candidate underlying the early maturity 5 locus in barley (Hordeum vulgare L.). We fine-mapped the gene using a mapping-by-sequencing approach applied on the whole-exome capture data from bulked early flowering segregants derived from a backcross of the Bowman(eam5) introgression line. We demonstrate that eam5 disrupts circadian expression of clock genes. Moreover, it interacts with the major photoperiod response gene Ppd-H1 to accelerate flowering under non-inductive short days. Our results suggest that HvPHYC participates in transmission of light signals to the circadian clock and thus modulates light-dependent processes such as photoperiodic regulation of flowering.",

author = "Artem Pankin and Chiara Campoli and Xue Dong and Benjamin Kilian and Rajiv Sharma and Axel Himmelbach and Reena Saini and Davis, {Seth J} and Nils Stein and Korbinian Schneeberger and {von Korff}, Maria",

note = "Copyright {\textcopyright} 2014, The Genetics Society of America.",

year = "2014",

month = sep,

day = "1",

doi = "10.1534/genetics.114.165613",

language = "English",

volume = "198",

pages = "383--396",

journal = "Human Genetics",

issn = "0340-6717",

publisher = "Springer",

number = "1",

}

Pankin, A, Campoli, C, Dong, X, Kilian, B, Sharma, R, Himmelbach, A, Saini, R, Davis, SJ, Stein, N, Schneeberger, K & von Korff, M 2014, 'Mapping-by-Sequencing Identifies HvPHYTOCHROME C as a Candidate Gene for the early maturity 5 Locus Modulating the Circadian Clock and Photoperiodic Flowering in Barley', Human Genetics, vol. 198, no. 1, pp. 383-396. https://doi.org/10.1534/genetics.114.165613

TY - JOUR

T1 - Mapping-by-Sequencing Identifies HvPHYTOCHROME C as a Candidate Gene for the early maturity 5 Locus Modulating the Circadian Clock and Photoperiodic Flowering in Barley

AU - Pankin, Artem

AU - Campoli, Chiara

AU - Dong, Xue

AU - Kilian, Benjamin

AU - Sharma, Rajiv

AU - Himmelbach, Axel

AU - Saini, Reena

AU - Davis, Seth J

AU - Stein, Nils

AU - Schneeberger, Korbinian

AU - von Korff, Maria

PY - 2014/9/1

Y1 - 2014/9/1

N2 - Phytochromes play an important role in light signaling and photoperiodic control of flowering time in plants. Here we propose that the red/far-red light photoreceptor HvPHYTOCHROME C (HvPHYC), carrying a mutation in a conserved region of the GAF domain, is a candidate underlying the early maturity 5 locus in barley (Hordeum vulgare L.). We fine-mapped the gene using a mapping-by-sequencing approach applied on the whole-exome capture data from bulked early flowering segregants derived from a backcross of the Bowman(eam5) introgression line. We demonstrate that eam5 disrupts circadian expression of clock genes. Moreover, it interacts with the major photoperiod response gene Ppd-H1 to accelerate flowering under non-inductive short days. Our results suggest that HvPHYC participates in transmission of light signals to the circadian clock and thus modulates light-dependent processes such as photoperiodic regulation of flowering.

AB - Phytochromes play an important role in light signaling and photoperiodic control of flowering time in plants. Here we propose that the red/far-red light photoreceptor HvPHYTOCHROME C (HvPHYC), carrying a mutation in a conserved region of the GAF domain, is a candidate underlying the early maturity 5 locus in barley (Hordeum vulgare L.). We fine-mapped the gene using a mapping-by-sequencing approach applied on the whole-exome capture data from bulked early flowering segregants derived from a backcross of the Bowman(eam5) introgression line. We demonstrate that eam5 disrupts circadian expression of clock genes. Moreover, it interacts with the major photoperiod response gene Ppd-H1 to accelerate flowering under non-inductive short days. Our results suggest that HvPHYC participates in transmission of light signals to the circadian clock and thus modulates light-dependent processes such as photoperiodic regulation of flowering.

U2 - 10.1534/genetics.114.165613

DO - 10.1534/genetics.114.165613

M3 - Article

C2 - 24996910

SN - 0340-6717

VL - 198

SP - 383

EP - 396

JO - Human Genetics

JF - Human Genetics

IS - 1

ER -