MASS-SPECTROMETRIC ANALYSIS OF CHITIN OLIGOSACCHARIDES PRODUCED BY RHIZOBIUM NODC PROTEIN IN ESCHERICHIA-COLI

E  KAMST; K M G M  VANDERDRIFT; Jane Elizabeth Thomas-Oates; B J J  LUGTENBERG; H P  SPAINK

MASS-SPECTROMETRIC ANALYSIS OF CHITIN OLIGOSACCHARIDES PRODUCED BY RHIZOBIUM NODC PROTEIN IN ESCHERICHIA-COLI

E KAMST, K M G M VANDERDRIFT, Jane Elizabeth Thomas-Oates, B J J LUGTENBERG, H P SPAINK

Chemistry

Research output: Contribution to journal › Comment/debate › peer-review

Abstract

A system for studying the in vivo activity of Rhizobium NodC protein in Escherichia coli has been developed, Using thin-layer chromatography, high-performance liquid chromatography, and mass spectrometry, we show that in this system R. leguminosarum by. viciae NodC protein directs the synthesis of chitinpentaose, chitintetraose, chitintriose, and two as yet unidentified modified chitin oligosaccharides.

Original language	English
Pages (from-to)	6282-6285
Number of pages	4
Journal	Journal of Bacteriology
Volume	177
Issue number	21
Publication status	Published - Nov 1995

Keywords

BIOSYNTHESIS
NODULATION

Cite this

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title = "MASS-SPECTROMETRIC ANALYSIS OF CHITIN OLIGOSACCHARIDES PRODUCED BY RHIZOBIUM NODC PROTEIN IN ESCHERICHIA-COLI",

abstract = "A system for studying the in vivo activity of Rhizobium NodC protein in Escherichia coli has been developed, Using thin-layer chromatography, high-performance liquid chromatography, and mass spectrometry, we show that in this system R. leguminosarum by. viciae NodC protein directs the synthesis of chitinpentaose, chitintetraose, chitintriose, and two as yet unidentified modified chitin oligosaccharides.",

keywords = "BIOSYNTHESIS, NODULATION",

author = "E KAMST and VANDERDRIFT, {K M G M} and Thomas-Oates, {Jane Elizabeth} and LUGTENBERG, {B J J} and SPAINK, {H P}",

year = "1995",

month = nov,

language = "English",

volume = "177",

pages = "6282--6285",

journal = "Journal of Bacteriology",

issn = "0021-9193",

publisher = "American Society for Microbiology",

number = "21",

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T1 - MASS-SPECTROMETRIC ANALYSIS OF CHITIN OLIGOSACCHARIDES PRODUCED BY RHIZOBIUM NODC PROTEIN IN ESCHERICHIA-COLI

AU - KAMST, E

AU - VANDERDRIFT, K M G M

AU - Thomas-Oates, Jane Elizabeth

AU - LUGTENBERG, B J J

AU - SPAINK, H P

PY - 1995/11

Y1 - 1995/11

N2 - A system for studying the in vivo activity of Rhizobium NodC protein in Escherichia coli has been developed, Using thin-layer chromatography, high-performance liquid chromatography, and mass spectrometry, we show that in this system R. leguminosarum by. viciae NodC protein directs the synthesis of chitinpentaose, chitintetraose, chitintriose, and two as yet unidentified modified chitin oligosaccharides.

AB - A system for studying the in vivo activity of Rhizobium NodC protein in Escherichia coli has been developed, Using thin-layer chromatography, high-performance liquid chromatography, and mass spectrometry, we show that in this system R. leguminosarum by. viciae NodC protein directs the synthesis of chitinpentaose, chitintetraose, chitintriose, and two as yet unidentified modified chitin oligosaccharides.

KW - BIOSYNTHESIS

KW - NODULATION

M3 - Comment/debate

SN - 0021-9193

VL - 177

SP - 6282

EP - 6285

JO - Journal of Bacteriology

JF - Journal of Bacteriology

IS - 21

ER -