Mass spectrometric analysis of lipo-chitin oligosaccharides - Signal molecules mediating the host-specific legume-rhizobium symbiosis

K M G M van der Drift, M M A Olsthoorn, L P Brull, L Blok-Tip, J E Thomas-Oates

Research output: Contribution to journalLiterature reviewpeer-review

Abstract

Lipo-chitin oligosaccharides (LCOs) are novel bacterial glycolipid signal molecules that mediate the species-specific symbiosis between rhizobial bacteria and leguminous plants. Nodulation of the legume roots and nitrogen-fixation in the resulting nodules by Rhizobia is controlled by the bacterial nodulation genes that encode the LCO biosynthetic enzymes. The length of the LCO chitin backbone, the length and degree of unsaturation of the fatty acyl chain attached to it, and the combination of different chemical substituents on the reducing- and nonreducing-terminal residues all contribute to the species-specificity of the signal. LCOs are bioactive in the nanomolar and subnanomolar concentration range and are produced as heterogeneous mixtures, making determination of their. structures a difficult task, most successfully approached by the application of modern mass spectrometric methods in combination with specific chemical treatments nir,zed at identifying specific chemical moieties. This review presents an overview of these methods as they are being used for the structural elucidation of LCOs, and discusses the role of structural diversity in mediating species-specificity. (C) 1999 John Wiley & Sons, Inc.

Original languageEnglish
Pages (from-to)75-95
Number of pages21
JournalMass Spectrometry Reviews
Volume17
Issue number2
Publication statusPublished - 1998

Keywords

  • CHITOLIPOOLIGOSACCHARIDE NOD FACTORS
  • DIMETHYL DISULFIDE DERIVATIVES
  • LEGUMINOSARUM BV TRIFOLII
  • FATTY ACYL MOIETIES
  • NODULATION FACTORS
  • PATHOGEN INTERACTIONS
  • STRUCTURAL DETERMINATION
  • HIGH-RESOLUTION
  • BRADYRHIZOBIUM-JAPONICUM
  • PLANT MORPHOGENESIS

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