Measuring Hypocotyl Length in Arabidopsis

James Ronald, Seth Jon Davis*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter


Circadian clocks allow organisms to synchronize growth to occur at the most optimal time of the day. In plants, the circadian clock controls the timing of hypocotyl (seedling stem) elongation. The activity of the circadian clock subsequently results in hypocotyl elongation being restricted to a small window around dawn and the early morning. Measuring hypocotyl elongation has provided circadian biologists a quick and non-intensive experimental tool to understand the effect of a circadian mutation on plant growth. Furthermore, hypocotyl elongation is also independently regulated by light, temperature, and hormone signaling pathways. Thus, hypocotyl assays can be expanded to investigate the crosstalk between the circadian clock and other extrinsic and intrinsic signaling pathways in controlling plant development. In this chapter we describe the resources and methods required to set up and analyze hypocotyl elongation in Arabidopsis.

Original languageEnglish
Title of host publicationPlant Circadian Networks Methods and Protocols
EditorsDorothy Staiger, Seth Davis, Amanda Melaragno Davis
Place of PublicationNew York
PublisherHumana Press Inc.
Number of pages8
ISBN (Electronic)978-1-0716-1912-4
ISBN (Print)978-1-0716-1911-7
Publication statusPublished - 2022

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Bibliographical note

Publisher Copyright:
© 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.


  • Circadian clock
  • Circadian mutants
  • Growth
  • Hormone signaling
  • Hypocotyl
  • Light signaling

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