Measuring nitric oxide metabolism in the pathogen Neisseria meningitidis

TY - CHAP

T1 - Measuring nitric oxide metabolism in the pathogen Neisseria meningitidis

AU - Thomson, Melanie J.

AU - Stevanin, Tania M.

AU - Moir, James W. B.

PY - 2008

Y1 - 2008

N2 - This chapter illustrates some of the considerations that need to be made when analyzing nitric oxide (NO) metabolism of the pathogen Neisseria meningitidis. These considerations are pertinent to other bacteria and, in particular, other pathogens. First, because of the small culture volumes that can generally be managed safely, culture experiments are maintained in small volumes. We show a method for simultaneously measuring oxygen and nitric oxide during growth of N. meningitidis in a bioreactor/electrode chamber adapted from commercially available equipment. NO and NO-generating compounds can be used to investigate the impact of NO on N. meningitidis metabolism and gene expression in pure culture. Also, methods are described for analyzing the role of NO during the interaction between N. meningitidis and human macrophage cells that generate NO.

AB - This chapter illustrates some of the considerations that need to be made when analyzing nitric oxide (NO) metabolism of the pathogen Neisseria meningitidis. These considerations are pertinent to other bacteria and, in particular, other pathogens. First, because of the small culture volumes that can generally be managed safely, culture experiments are maintained in small volumes. We show a method for simultaneously measuring oxygen and nitric oxide during growth of N. meningitidis in a bioreactor/electrode chamber adapted from commercially available equipment. NO and NO-generating compounds can be used to investigate the impact of NO on N. meningitidis metabolism and gene expression in pure culture. Also, methods are described for analyzing the role of NO during the interaction between N. meningitidis and human macrophage cells that generate NO.

KW - OUTER-MEMBRANE PROTEIN

KW - HUMAN MACROPHAGES

KW - DENITRIFICATION

KW - GONORRHOEAE

KW - EXPRESSION

KW - REPRESSOR

KW - BACTERIA

KW - NITRATE

KW - NSRR

KW - REDUCTASE

UR - http://www.scopus.com/inward/record.url?scp=47049086638&partnerID=8YFLogxK

U2 - 10.1016/S0076-6879(07)37027-4

DO - 10.1016/S0076-6879(07)37027-4

M3 - Other chapter contribution

SN - 9780080877921

SP - 539

EP - 560

BT - GLOBINS AND OTHER NITRIC OXIDE-REACTIVE PROTEINS, PART B

PB - Elsevier

CY - SAN DIEGO

ER -