Measuring Non-canonical Inflammasome Activation in Neutrophils

Mercedes Monteleone; Dave Boucher

doi:10.1007/978-1-0716-2144-8_3

Measuring Non-canonical Inflammasome Activation in Neutrophils

Mercedes Monteleone, Dave Boucher

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Neutrophils are innate immune cells that play critical functions during infections through diverse mechanisms. One such mechanism, the generation of extracellular traps (NETs), enables direct bacterial killing during infections. We recently reported that the activation of the non-canonical inflammasomes in neutrophils allows for the generation of NETs and is an important host defence mechanism in vivo in response to intracellular Gram-negative bacterium. This process is dependent on inflammatory caspases and the cell death effector Gasdermin D. Here, we describe a simple approach to study the functions of the non-canonical inflammasome in murine neutrophils using microscopy and cellular fragmentation assays.

Original language	English
Pages (from-to)	29-37
Number of pages	9
Journal	Methods in Molecular Biology
Volume	2459
DOIs	https://doi.org/10.1007/978-1-0716-2144-8_3
Publication status	Published - 25 Feb 2022

Bibliographical note

Access to Document

10.1007/978-1-0716-2144-8_3

Cite this

@article{0d8ba37e125e41bbabba0a80a45be3b4,

title = "Measuring Non-canonical Inflammasome Activation in Neutrophils",

abstract = "Neutrophils are innate immune cells that play critical functions during infections through diverse mechanisms. One such mechanism, the generation of extracellular traps (NETs), enables direct bacterial killing during infections. We recently reported that the activation of the non-canonical inflammasomes in neutrophils allows for the generation of NETs and is an important host defence mechanism in vivo in response to intracellular Gram-negative bacterium. This process is dependent on inflammatory caspases and the cell death effector Gasdermin D. Here, we describe a simple approach to study the functions of the non-canonical inflammasome in murine neutrophils using microscopy and cellular fragmentation assays.",

author = "Mercedes Monteleone and Dave Boucher",

note = "{\textcopyright} 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",

year = "2022",

month = feb,

day = "25",

doi = "10.1007/978-1-0716-2144-8_3",

language = "English",

volume = "2459",

pages = "29--37",

journal = "Methods in Molecular Biology",

issn = "1064-3745",

publisher = "Humana Press",

}

TY - JOUR

T1 - Measuring Non-canonical Inflammasome Activation in Neutrophils

AU - Monteleone, Mercedes

AU - Boucher, Dave

PY - 2022/2/25

Y1 - 2022/2/25

N2 - Neutrophils are innate immune cells that play critical functions during infections through diverse mechanisms. One such mechanism, the generation of extracellular traps (NETs), enables direct bacterial killing during infections. We recently reported that the activation of the non-canonical inflammasomes in neutrophils allows for the generation of NETs and is an important host defence mechanism in vivo in response to intracellular Gram-negative bacterium. This process is dependent on inflammatory caspases and the cell death effector Gasdermin D. Here, we describe a simple approach to study the functions of the non-canonical inflammasome in murine neutrophils using microscopy and cellular fragmentation assays.

AB - Neutrophils are innate immune cells that play critical functions during infections through diverse mechanisms. One such mechanism, the generation of extracellular traps (NETs), enables direct bacterial killing during infections. We recently reported that the activation of the non-canonical inflammasomes in neutrophils allows for the generation of NETs and is an important host defence mechanism in vivo in response to intracellular Gram-negative bacterium. This process is dependent on inflammatory caspases and the cell death effector Gasdermin D. Here, we describe a simple approach to study the functions of the non-canonical inflammasome in murine neutrophils using microscopy and cellular fragmentation assays.

U2 - 10.1007/978-1-0716-2144-8_3

DO - 10.1007/978-1-0716-2144-8_3

M3 - Article

C2 - 35212951

SN - 1064-3745

VL - 2459

SP - 29

EP - 37

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -