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Measuring Non-canonical Inflammasome Activation in Neutrophils

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JournalMethods in Molecular Biology
DatePublished - 1 Jan 2022
Volume2459
Number of pages9
Pages (from-to)29-37
Original languageEnglish

Abstract

Neutrophils are innate immune cells that play critical functions during infections through diverse mechanisms. One such mechanism, the generation of extracellular traps (NETs), enables direct bacterial killing during infections. We recently reported that the activation of the non-canonical inflammasomes in neutrophils allows for the generation of NETs and is an important host defence mechanism in vivo in response to intracellular Gram-negative bacterium. This process is dependent on inflammatory caspases and the cell death effector Gasdermin D. Here, we describe a simple approach to study the functions of the non-canonical inflammasome in murine neutrophils using microscopy and cellular fragmentation assays.

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© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

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