Mechanistic insights into glycosidase chemistry

TY - JOUR

T1 - Mechanistic insights into glycosidase chemistry

AU - Vocadlo, David J.

AU - Davies, Gideon J.

PY - 2008/10

Y1 - 2008/10

N2 - The enzymatic hydrolysis of the glycosidic bond continues to gain importance, reflecting the critically important roles complex glycans play in health and disease as well as the rekindled interest in enzymatic biomass conversion. Recent advances include the broadening of our understanding of enzyme reaction coordinates, through both computational and structural studies, improved understanding of enzyme inhibition through transition state mimicry and fascinating insights into mechanism yielded by physical organic chemistry approaches.

AB - The enzymatic hydrolysis of the glycosidic bond continues to gain importance, reflecting the critically important roles complex glycans play in health and disease as well as the rekindled interest in enzymatic biomass conversion. Recent advances include the broadening of our understanding of enzyme reaction coordinates, through both computational and structural studies, improved understanding of enzyme inhibition through transition state mimicry and fascinating insights into mechanism yielded by physical organic chemistry approaches.

KW - TRANSITION-STATE ANALOGS

KW - BETA-N-ACETYLGLUCOSAMINIDASES

KW - ALPHA-MANNOSIDASE-II

KW - COVALENT INTERMEDIATE

KW - KINETIC-ANALYSIS

KW - O-GLCNACASE

KW - CATALYTIC MECHANISM

KW - THERMOTOGA-MARITIMA

KW - CRYSTAL-STRUCTURE

KW - STRUCTURAL BASIS

UR - http://www.scopus.com/inward/record.url?scp=53849121583&partnerID=8YFLogxK

U2 - 10.1016/j.cbpa.2008.05.010

DO - 10.1016/j.cbpa.2008.05.010

M3 - Literature review

SN - 1367-5931

VL - 12

SP - 539

EP - 555

JO - Current Opinion in Chemical Biology

JF - Current Opinion in Chemical Biology

IS - 5

ER -