Membrane electroporation theories: a review

C. Chen; S. W. Smye; M. P. Robinson; J. A. Evans

doi:10.1007/s11517-005-0020-2

Membrane electroporation theories: a review

C. Chen, S. W. Smye, M. P. Robinson, J. A. Evans

Electronic Engineering

Research output: Contribution to journal › Literature review › peer-review

Abstract

Electroporation, the transient increase in the permeability of cell membranes when exposed to a high electric field, is an established in vitro technique and is used to introduce DNA or other molecules into cells. When the trans-membrane voltage induced by an external electric field exceeds a certain threshold (normally 0.2-1 V), a rearrangement of the molecular structure of the membrane occurs, leading to pore formation in the membrane and a considerable increase in the cell membrane permeability to ions, molecules and even macromolecules. This phenomenon is, potentially, the basis for many in vivo applications such as electrochemotherapy and gene therapy, but still lacks a comprehensive theoretical basis. This article reviews the state of current electroporation theories and briefly considers current and potential applications in biology and medicine.

Original language	English
Pages (from-to)	5-14
Number of pages	10
Journal	Medical and Biological Engineering and Computing
Volume	44
Issue number	1-2
DOIs	https://doi.org/10.1007/s11517-005-0020-2
Publication status	Published - Mar 2006

Keywords

electroporation
electropermeabilisation
cell membrane
aqueous pore model
electric fields
REVERSIBLE ELECTRICAL BREAKDOWN
PLANAR BILAYER-MEMBRANES
IN-VIVO ELECTROPORATION
LIPID-BILAYERS
CELL-MEMBRANES
FIELD
STABILITY
MODEL
ELECTROPERMEABILIZATION
SIMULATION

Access to Document

10.1007/s11517-005-0020-2

Cite this

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title = "Membrane electroporation theories: a review",

abstract = "Electroporation, the transient increase in the permeability of cell membranes when exposed to a high electric field, is an established in vitro technique and is used to introduce DNA or other molecules into cells. When the trans-membrane voltage induced by an external electric field exceeds a certain threshold (normally 0.2-1 V), a rearrangement of the molecular structure of the membrane occurs, leading to pore formation in the membrane and a considerable increase in the cell membrane permeability to ions, molecules and even macromolecules. This phenomenon is, potentially, the basis for many in vivo applications such as electrochemotherapy and gene therapy, but still lacks a comprehensive theoretical basis. This article reviews the state of current electroporation theories and briefly considers current and potential applications in biology and medicine.",

keywords = "electroporation, electropermeabilisation, cell membrane, aqueous pore model, electric fields, REVERSIBLE ELECTRICAL BREAKDOWN, PLANAR BILAYER-MEMBRANES, IN-VIVO ELECTROPORATION, LIPID-BILAYERS, CELL-MEMBRANES, FIELD, STABILITY, MODEL, ELECTROPERMEABILIZATION, SIMULATION",

author = "C. Chen and Smye, {S. W.} and Robinson, {M. P.} and Evans, {J. A.}",

year = "2006",

month = mar,

doi = "10.1007/s11517-005-0020-2",

language = "English",

volume = "44",

pages = "5--14",

journal = "Medical and Biological Engineering and Computing",

issn = "0140-0118",

publisher = "Springer",

number = "1-2",

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TY - JOUR

T1 - Membrane electroporation theories

T2 - a review

AU - Chen, C.

AU - Smye, S. W.

AU - Robinson, M. P.

AU - Evans, J. A.

PY - 2006/3

Y1 - 2006/3

N2 - Electroporation, the transient increase in the permeability of cell membranes when exposed to a high electric field, is an established in vitro technique and is used to introduce DNA or other molecules into cells. When the trans-membrane voltage induced by an external electric field exceeds a certain threshold (normally 0.2-1 V), a rearrangement of the molecular structure of the membrane occurs, leading to pore formation in the membrane and a considerable increase in the cell membrane permeability to ions, molecules and even macromolecules. This phenomenon is, potentially, the basis for many in vivo applications such as electrochemotherapy and gene therapy, but still lacks a comprehensive theoretical basis. This article reviews the state of current electroporation theories and briefly considers current and potential applications in biology and medicine.

AB - Electroporation, the transient increase in the permeability of cell membranes when exposed to a high electric field, is an established in vitro technique and is used to introduce DNA or other molecules into cells. When the trans-membrane voltage induced by an external electric field exceeds a certain threshold (normally 0.2-1 V), a rearrangement of the molecular structure of the membrane occurs, leading to pore formation in the membrane and a considerable increase in the cell membrane permeability to ions, molecules and even macromolecules. This phenomenon is, potentially, the basis for many in vivo applications such as electrochemotherapy and gene therapy, but still lacks a comprehensive theoretical basis. This article reviews the state of current electroporation theories and briefly considers current and potential applications in biology and medicine.

KW - electroporation

KW - electropermeabilisation

KW - cell membrane

KW - aqueous pore model

KW - electric fields

KW - REVERSIBLE ELECTRICAL BREAKDOWN

KW - PLANAR BILAYER-MEMBRANES

KW - IN-VIVO ELECTROPORATION

KW - LIPID-BILAYERS

KW - CELL-MEMBRANES

KW - FIELD

KW - STABILITY

KW - MODEL

KW - ELECTROPERMEABILIZATION

KW - SIMULATION

U2 - 10.1007/s11517-005-0020-2

DO - 10.1007/s11517-005-0020-2

M3 - Literature review

SN - 0140-0118

VL - 44

SP - 5

EP - 14

JO - Medical and Biological Engineering and Computing

JF - Medical and Biological Engineering and Computing

IS - 1-2

ER -