Metabolite Identification of Helicobacter Pylori Supernatant Using Near-IR Raman Spectroscopy

M Chauhan; A Buckley; A Jha; M Wilcox; V Subramaniam; P Valdastri

doi:10.1109/icton51198.2020.9203521

Metabolite Identification of Helicobacter Pylori Supernatant Using Near-IR Raman Spectroscopy

M Chauhan, A Buckley, A Jha, M Wilcox, V Subramaniam, P Valdastri

Electronic Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Helicobacter pylori, a non-invasive gram-negative bacteria is well known for its direct cause and effect relationship with peptic ulcer, gastric or duodenal ulceration. Its severe effect on human begin with its colonization and production of various enzyme like Vacuolating cytotoxin, Urease and Heat shock proteins. Urease is complex spherical protein which causes hydrolysis of gastric urea to ammonia and CO 2 . The enzymatic reaction results in increase of gastric pH and hence results in gastric cancer. We present a photonic based technique to identify these proteins in its natural supernatant condition. The Raman spectrum of Helicobacter pylori is reported and the vibrational peaks are identified with corresponding chemical bonds in the biochemical enzyme. This can lay the foundation for future diagnostic tool for clinical detection of peptic ulcer, gastric or duodenal ulceration.

Original language	English
Title of host publication	2020 22nd International Conference on Transparent Optical Networks (ICTON)
Publisher	IEEE
ISBN (Print)	9781728184241
DOIs	https://doi.org/10.1109/icton51198.2020.9203521
Publication status	Published - 22 Sept 2020

Access to Document

10.1109/icton51198.2020.9203521

Cite this

@inproceedings{bfbf73cdd8d74482b65648e35ab8a1a7,

title = "Metabolite Identification of Helicobacter Pylori Supernatant Using Near-IR Raman Spectroscopy",

abstract = "Helicobacter pylori, a non-invasive gram-negative bacteria is well known for its direct cause and effect relationship with peptic ulcer, gastric or duodenal ulceration. Its severe effect on human begin with its colonization and production of various enzyme like Vacuolating cytotoxin, Urease and Heat shock proteins. Urease is complex spherical protein which causes hydrolysis of gastric urea to ammonia and CO 2 . The enzymatic reaction results in increase of gastric pH and hence results in gastric cancer. We present a photonic based technique to identify these proteins in its natural supernatant condition. The Raman spectrum of Helicobacter pylori is reported and the vibrational peaks are identified with corresponding chemical bonds in the biochemical enzyme. This can lay the foundation for future diagnostic tool for clinical detection of peptic ulcer, gastric or duodenal ulceration.",

author = "M Chauhan and A Buckley and A Jha and M Wilcox and V Subramaniam and P Valdastri",

year = "2020",

month = sep,

day = "22",

doi = "10.1109/icton51198.2020.9203521",

language = "English",

isbn = "9781728184241",

booktitle = "2020 22nd International Conference on Transparent Optical Networks (ICTON)",

publisher = "IEEE",

address = "United States",

}

TY - GEN

T1 - Metabolite Identification of Helicobacter Pylori Supernatant Using Near-IR Raman Spectroscopy

AU - Chauhan, M

AU - Buckley, A

AU - Jha, A

AU - Wilcox, M

AU - Subramaniam, V

AU - Valdastri, P

PY - 2020/9/22

Y1 - 2020/9/22

N2 - Helicobacter pylori, a non-invasive gram-negative bacteria is well known for its direct cause and effect relationship with peptic ulcer, gastric or duodenal ulceration. Its severe effect on human begin with its colonization and production of various enzyme like Vacuolating cytotoxin, Urease and Heat shock proteins. Urease is complex spherical protein which causes hydrolysis of gastric urea to ammonia and CO 2 . The enzymatic reaction results in increase of gastric pH and hence results in gastric cancer. We present a photonic based technique to identify these proteins in its natural supernatant condition. The Raman spectrum of Helicobacter pylori is reported and the vibrational peaks are identified with corresponding chemical bonds in the biochemical enzyme. This can lay the foundation for future diagnostic tool for clinical detection of peptic ulcer, gastric or duodenal ulceration.

AB - Helicobacter pylori, a non-invasive gram-negative bacteria is well known for its direct cause and effect relationship with peptic ulcer, gastric or duodenal ulceration. Its severe effect on human begin with its colonization and production of various enzyme like Vacuolating cytotoxin, Urease and Heat shock proteins. Urease is complex spherical protein which causes hydrolysis of gastric urea to ammonia and CO 2 . The enzymatic reaction results in increase of gastric pH and hence results in gastric cancer. We present a photonic based technique to identify these proteins in its natural supernatant condition. The Raman spectrum of Helicobacter pylori is reported and the vibrational peaks are identified with corresponding chemical bonds in the biochemical enzyme. This can lay the foundation for future diagnostic tool for clinical detection of peptic ulcer, gastric or duodenal ulceration.

U2 - 10.1109/icton51198.2020.9203521

DO - 10.1109/icton51198.2020.9203521

M3 - Conference contribution

SN - 9781728184241

BT - 2020 22nd International Conference on Transparent Optical Networks (ICTON)

PB - IEEE

ER -