Abstract
Hydrophilic interaction liquid chromatography (HILIC), although not a new technique, has enjoyed a recent renaissance with the introduction of robust and reproducible stationary phases. It is consequently finding application in metabolomics studies, which have traditionally relied on the stability of reversed phases (RPs), since the biofluids analyzed are predominantly aqueous and thus contain many polar analytes. HILIC's retention of those polar compounds and use of solvents readily compatible with mass spectrometry have seen its increasing adoption in studies of complex aqueous metabolomes. This review describes the stationary phases and their features. surveys HILIC-LC-MS's role in metabolomics experiments, discusses approaches to data extraction and analysis including multivariate analysis, and reviews the literature on HILIC MS applications in metabolomics. (C) 2009 Wiley Periodicals, Inc., Mass Spec Rev 29:671-684, 2010
| Original language | English |
|---|---|
| Pages (from-to) | 671-684 |
| Number of pages | 14 |
| Journal | Mass Spectrometry Reviews |
| Volume | 29 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - Sept 2010 |
Keywords
- hydrophilic interaction liquid chromatography (HILIC)
- LC-MS
- metabolomics
- metabonomics
- plant metabolites
- urine
- principle component analysis (PCA)
- multivariate analysis (MVA)
- HYDROPHILIC-INTERACTION CHROMATOGRAPHY
- TANDEM MASS-SPECTROMETRY
- PERFORMANCE LIQUID-CHROMATOGRAPHY
- MULTIVARIATE STATISTICAL-ANALYSIS
- SMALL POLAR COMPOUNDS
- HUMAN PLASMA
- PRINCIPAL COMPONENTS
- BIOLOGICAL-FLUIDS
- SYSTEMS BIOLOGY
- SILICA COLUMNS