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Methodologies for “Wiring” Redox Proteins/Enzymes to Electrode Surfaces

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JournalChemistry : A European Journal
DateAccepted/In press - 10 Apr 2018
DatePublished (current) - 10 Apr 2018
Pages (from-to)1-20
Original languageEnglish


The immobilization of redox proteins or enzymes onto conductive surfaces has application in the analysis of biological processes, the fabrication of biosensors, and in the development of green technologies and biochemical synthetic approaches. This review evaluates the methods through which redox proteins can be attached to electrode surfaces in a “wired” configuration, that is, one that facilitates direct electron transfer. The feasibility of simple electroactive adsorption onto a range of electrode surfaces is illustrated, with a highlight on the recent advances that have been achieved in biotechnological device construction using carbon materials and metal oxides. The covalent crosslinking strategies commonly used for the modification and biofunctionalization of electrode surfaces are also evaluated. Recent innovations in harnessing chemical biology methods for electrically wiring redox biology to surfaces are emphasized.

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©2018 The Authors.

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