By the same authors

Microalgae Isoprostanoids Profiling: Species Choice and Growing Conditions Impact

Research output: Contribution to conferencePosterpeer-review


  • Claire Vigor
  • Guillaume Reversat
  • Amandine Rocher
  • A. Linares-Maurizi
  • Camille Oger
  • Jean-Marie Galano
  • Joseph Vercauteren
  • Thierry Durand
  • Catherine Leblanc
  • P. Potin
  • Thierry Tonon



Conference17th Euro Fed Lipid Congress
Conference date(s)20/10/1923/10/19
Internet address

Publication details

DatePublished - 20 Oct 2019
Original languageEnglish


lgae result from a complex evolutionary history that shape their metabolic network. Indeed, they are able to synthesize a-linolenic acid (ALA) as terrestrial plant, but also eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA) that are found in oily fish. Notably, some microalgae are the principal producers of long-chain polyunsaturated fatty acids (LC-PUFAs) in marine ecosystems. Importantly, due to the presence of numerous double bonds, LC-PUFAs are readily oxidized, via a non-enzymatic pathway for instance, leading to the formation of high added-value metabolites named isoprostanoids. These molecules have been shown to display a wide range of biological properties (e.g. anti-inflammatory, neuroprotective, and anti-arrhythmic).

Our project was based on four microalgae selected according to their LC-PUFAs profile and representing different algal lineages, and comprised two aspects: i) determination of the isoprostanoid baseline level of species grown under normal conditions to investigate the postulate "high level of parent LC-PUFA corresponds to high level of associated oxidized metabolites"; ii) evaluation of the impact of individual oxidative stress (Cu2+, H2O2) on the of isoprostanoid content.

Preliminary data of isoprostanoids quantified by a micro-LC-MS/MS method indicated the existence of a correlation between the presence of LC-PUFAs and the biosynthesis of related oxidized metabolites, except for Rhodomonas salina that is rich in EPA and DHA but shows high amount of C18- (ALA) derived isoprostanoids. After exposure to potential oxidative stress, changes in content and profile of LC-PUFA derived oxidized metabolites were species and stress dependent. No variations were observed in Rhodomonas salina and Chaetoceros gracilis, whereas an increase in the production of C18-, C20- and C22- derived isoprostanoids was monitored in Tisochrysis lutea under Cu2+ stress. A similar pattern was observed in Phaeodactlyum tricornutum. However, and unexpectedly, the production of several isoprostanoids in this alga decreased after H2O2 stress compared to control conditions.

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