Abstract
lgae result from a complex evolutionary history that shape their metabolic network. Indeed, they are able to synthesize a-linolenic acid (ALA) as terrestrial plant, but also eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA) that are found in oily fish. Notably, some microalgae are the principal producers of long-chain polyunsaturated fatty acids (LC-PUFAs) in marine ecosystems. Importantly, due to the presence of numerous double bonds, LC-PUFAs are readily oxidized, via a non-enzymatic pathway for instance, leading to the formation of high added-value metabolites named isoprostanoids. These molecules have been shown to display a wide range of biological properties (e.g. anti-inflammatory, neuroprotective, and anti-arrhythmic).
Our project was based on four microalgae selected according to their LC-PUFAs profile and representing different algal lineages, and comprised two aspects: i) determination of the isoprostanoid baseline level of species grown under normal conditions to investigate the postulate "high level of parent LC-PUFA corresponds to high level of associated oxidized metabolites"; ii) evaluation of the impact of individual oxidative stress (Cu2+, H2O2) on the of isoprostanoid content.
Preliminary data of isoprostanoids quantified by a micro-LC-MS/MS method indicated the existence of a correlation between the presence of LC-PUFAs and the biosynthesis of related oxidized metabolites, except for Rhodomonas salina that is rich in EPA and DHA but shows high amount of C18- (ALA) derived isoprostanoids. After exposure to potential oxidative stress, changes in content and profile of LC-PUFA derived oxidized metabolites were species and stress dependent. No variations were observed in Rhodomonas salina and Chaetoceros gracilis, whereas an increase in the production of C18-, C20- and C22- derived isoprostanoids was monitored in Tisochrysis lutea under Cu2+ stress. A similar pattern was observed in Phaeodactlyum tricornutum. However, and unexpectedly, the production of several isoprostanoids in this alga decreased after H2O2 stress compared to control conditions.
Our project was based on four microalgae selected according to their LC-PUFAs profile and representing different algal lineages, and comprised two aspects: i) determination of the isoprostanoid baseline level of species grown under normal conditions to investigate the postulate "high level of parent LC-PUFA corresponds to high level of associated oxidized metabolites"; ii) evaluation of the impact of individual oxidative stress (Cu2+, H2O2) on the of isoprostanoid content.
Preliminary data of isoprostanoids quantified by a micro-LC-MS/MS method indicated the existence of a correlation between the presence of LC-PUFAs and the biosynthesis of related oxidized metabolites, except for Rhodomonas salina that is rich in EPA and DHA but shows high amount of C18- (ALA) derived isoprostanoids. After exposure to potential oxidative stress, changes in content and profile of LC-PUFA derived oxidized metabolites were species and stress dependent. No variations were observed in Rhodomonas salina and Chaetoceros gracilis, whereas an increase in the production of C18-, C20- and C22- derived isoprostanoids was monitored in Tisochrysis lutea under Cu2+ stress. A similar pattern was observed in Phaeodactlyum tricornutum. However, and unexpectedly, the production of several isoprostanoids in this alga decreased after H2O2 stress compared to control conditions.
| Original language | English |
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| Publication status | Published - 20 Oct 2019 |
| Event | 17th Euro Fed Lipid Congress - Seville, Spain Duration: 20 Oct 2019 → 23 Oct 2019 http://www.eurofedlipid.org/pages/sevilla.html |
Conference
| Conference | 17th Euro Fed Lipid Congress |
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| Country/Territory | Spain |
| City | Seville |
| Period | 20/10/19 → 23/10/19 |
| Internet address |