Microarray analysis identifies genes preferentially expressed in the lung schistosomulum of Schistosoma mansoni

G P Dillon; T Feltwell; J P Skelton; P D Ashton; P S Coulson; M A Quail; N Nikolaidou-Katsaridou; R A Wilson; A C Ivens

doi:10.1016/j.ijpara.2005.10.008

Microarray analysis identifies genes preferentially expressed in the lung schistosomulum of Schistosoma mansoni

G P Dillon, T Feltwell, J P Skelton, P D Ashton, P S Coulson, M A Quail, N Nikolaidou-Katsaridou, R A Wilson, A C Ivens

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

The lung schistosomulum of Schistosoma mansoni is a validated target of protective immunity elicited in vaccinated mice. To identify genes expressed at this stage we constructed a microarray, representing 3088 contigs and singlets, with cDNA derived from in vitro cultured larvae and used it to screen RNA from seven life-cycle stages. Clustering of genes by expression profile across the life cycle revealed a number of membrane, membrane-associated and secreted proteins up-regulated at the lung stage, that may represent potential immune targets. Two promising secreted molecules have homology to antigens with vaccine and/or immunomodulatory potential in other helminths. (c) 2005 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.

Original language	English
Pages (from-to)	18
Number of pages	8
Journal	International Journal for Parasitology
Volume	36
Issue number	1
DOIs	https://doi.org/10.1016/j.ijpara.2005.10.008
Publication status	Published - Jan 2006

Keywords

blood fluke
life cycle
transcriptome
secretion
membrane proteins
Schistosoma
CULTIVATION
INFECTION
MIGRATION
PARASITE
VACCINE
PROTEIN

Access to Document

10.1016/j.ijpara.2005.10.008

Cite this

@article{5a30d6219ecc48bbbb0b010c3ba8b400,

title = "Microarray analysis identifies genes preferentially expressed in the lung schistosomulum of Schistosoma mansoni",

abstract = "The lung schistosomulum of Schistosoma mansoni is a validated target of protective immunity elicited in vaccinated mice. To identify genes expressed at this stage we constructed a microarray, representing 3088 contigs and singlets, with cDNA derived from in vitro cultured larvae and used it to screen RNA from seven life-cycle stages. Clustering of genes by expression profile across the life cycle revealed a number of membrane, membrane-associated and secreted proteins up-regulated at the lung stage, that may represent potential immune targets. Two promising secreted molecules have homology to antigens with vaccine and/or immunomodulatory potential in other helminths. (c) 2005 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.",

keywords = "blood fluke, life cycle, transcriptome, secretion, membrane proteins, Schistosoma, CULTIVATION, INFECTION, MIGRATION, PARASITE, VACCINE, PROTEIN",

author = "Dillon, {G P} and T Feltwell and Skelton, {J P} and Ashton, {P D} and Coulson, {P S} and Quail, {M A} and N Nikolaidou-Katsaridou and Wilson, {R A} and Ivens, {A C}",

year = "2006",

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doi = "10.1016/j.ijpara.2005.10.008",

language = "English",

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AU - Dillon, G P

AU - Feltwell, T

AU - Skelton, J P

AU - Ashton, P D

AU - Coulson, P S

AU - Quail, M A

AU - Nikolaidou-Katsaridou, N

AU - Wilson, R A

AU - Ivens, A C

PY - 2006/1

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AB - The lung schistosomulum of Schistosoma mansoni is a validated target of protective immunity elicited in vaccinated mice. To identify genes expressed at this stage we constructed a microarray, representing 3088 contigs and singlets, with cDNA derived from in vitro cultured larvae and used it to screen RNA from seven life-cycle stages. Clustering of genes by expression profile across the life cycle revealed a number of membrane, membrane-associated and secreted proteins up-regulated at the lung stage, that may represent potential immune targets. Two promising secreted molecules have homology to antigens with vaccine and/or immunomodulatory potential in other helminths. (c) 2005 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.

KW - blood fluke

KW - life cycle

KW - transcriptome

KW - secretion

KW - membrane proteins

KW - Schistosoma

KW - CULTIVATION

KW - INFECTION

KW - MIGRATION

KW - PARASITE

KW - VACCINE

KW - PROTEIN

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DO - 10.1016/j.ijpara.2005.10.008

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SP - 18

JO - International Journal for Parasitology

JF - International Journal for Parasitology

SN - 0020-7519

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