The lung schistosomulum of Schistosoma mansoni is a validated target of protective immunity elicited in vaccinated mice. To identify genes expressed at this stage we constructed a microarray, representing 3088 contigs and singlets, with cDNA derived from in vitro cultured larvae and used it to screen RNA from seven life-cycle stages. Clustering of genes by expression profile across the life cycle revealed a number of membrane, membrane-associated and secreted proteins up-regulated at the lung stage, that may represent potential immune targets. Two promising secreted molecules have homology to antigens with vaccine and/or immunomodulatory potential in other helminths. (c) 2005 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.
|Number of pages||8|
|Journal||International Journal for Parasitology|
|Publication status||Published - Jan 2006|
- blood fluke
- life cycle
- membrane proteins