Microscale vapour diffusion for protein crystallization

Justyna Korczynska; Ting-Chou Hu; David K. Smith; Joby Jenkins; Rob Lewis; Tom Edwards; Andrzej M. Brzozowski

doi:10.1107/S0907444907037857

Microscale vapour diffusion for protein crystallization

Justyna Korczynska, Ting-Chou Hu, David K. Smith, Joby Jenkins, Rob Lewis, Tom Edwards, Andrzej M. Brzozowski

Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

The development of new crystallization platforms via the application of high-throughput technologies has delivered a plethora of crystallization plates suitable for robot-driven and manual setups. However, practically all these plates (except for microfluidic channel chips) are based on a very similar design and well (precipitant):drop (protein) volume ratios. A new type of crystallization plate (mu plate) has therefore been developed and tested that still employs the classical vapour-diffusion technique but minimizes the precipitant well volume to 1.2 mu l for a 150 nl protein drop setup. This enables a very significant saving on the total bulk of the crystallization screen, hence allowing the application of new, rare and expensive solutions in automated crystallization-screening procedures. Additionally, owing to the very low drop:well volume ratio, the new mu plate can significantly accelerate the equilibrium time necessary for crystal nucleation and growth, in many cases shortening the high-throughput crystallization screening process to a few hours.

Original language	English
Pages (from-to)	1009-1015
Number of pages	7
Journal	Acta Crystallographica. Section D, Biological Crystallography
Volume	63
DOIs	https://doi.org/10.1107/S0907444907037857
Publication status	Published - Sept 2007

Keywords

WATER EQUILIBRATION
DEPENDENCE
SPACE

Access to Document

10.1107/S0907444907037857

Cite this

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title = "Microscale vapour diffusion for protein crystallization",

abstract = "The development of new crystallization platforms via the application of high-throughput technologies has delivered a plethora of crystallization plates suitable for robot-driven and manual setups. However, practically all these plates (except for microfluidic channel chips) are based on a very similar design and well (precipitant):drop (protein) volume ratios. A new type of crystallization plate (mu plate) has therefore been developed and tested that still employs the classical vapour-diffusion technique but minimizes the precipitant well volume to 1.2 mu l for a 150 nl protein drop setup. This enables a very significant saving on the total bulk of the crystallization screen, hence allowing the application of new, rare and expensive solutions in automated crystallization-screening procedures. Additionally, owing to the very low drop:well volume ratio, the new mu plate can significantly accelerate the equilibrium time necessary for crystal nucleation and growth, in many cases shortening the high-throughput crystallization screening process to a few hours.",

keywords = "WATER EQUILIBRATION, DEPENDENCE, SPACE",

author = "Justyna Korczynska and Ting-Chou Hu and Smith, {David K.} and Joby Jenkins and Rob Lewis and Tom Edwards and Brzozowski, {Andrzej M.}",

year = "2007",

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T1 - Microscale vapour diffusion for protein crystallization

AU - Korczynska, Justyna

AU - Hu, Ting-Chou

AU - Smith, David K.

AU - Jenkins, Joby

AU - Lewis, Rob

AU - Edwards, Tom

AU - Brzozowski, Andrzej M.

PY - 2007/9

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AB - The development of new crystallization platforms via the application of high-throughput technologies has delivered a plethora of crystallization plates suitable for robot-driven and manual setups. However, practically all these plates (except for microfluidic channel chips) are based on a very similar design and well (precipitant):drop (protein) volume ratios. A new type of crystallization plate (mu plate) has therefore been developed and tested that still employs the classical vapour-diffusion technique but minimizes the precipitant well volume to 1.2 mu l for a 150 nl protein drop setup. This enables a very significant saving on the total bulk of the crystallization screen, hence allowing the application of new, rare and expensive solutions in automated crystallization-screening procedures. Additionally, owing to the very low drop:well volume ratio, the new mu plate can significantly accelerate the equilibrium time necessary for crystal nucleation and growth, in many cases shortening the high-throughput crystallization screening process to a few hours.

KW - WATER EQUILIBRATION

KW - DEPENDENCE

KW - SPACE

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