Mitochondrial DNA barcoding detects some species that are real, and some that are not

Kanchon K. Dasmahapatra; Marianne Elias; Ryan I. Hill; Joseph I. Hoffman; James Mallet

doi:10.1111/j.1755-0998.2009.02763.x

Mitochondrial DNA barcoding detects some species that are real, and some that are not

Kanchon K. Dasmahapatra, Marianne Elias, Ryan I. Hill, Joseph I. Hoffman, James Mallet

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Mimicry and extensive geographical subspecies polymorphism combine to make species in the ithomiine butterfly genus Mechanitis (Lepidoptera; Nymphalidae) difficult to determine. We use mitochondrial DNA (mtDNA) barcoding, nuclear sequences and amplified fragment length polymorphism (AFLP) genotyping to investigate species limits in this genus. Although earlier biosystematic studies based on morphology described only four species, mtDNA barcoding revealed eight well-differentiated haplogroups, suggesting the presence of four new putative 'cryptic species'. However, AFLP markers supported only one of these four new 'cryptic species' as biologically meaningful. We demonstrate that in this genus, deep genetic divisions expected on the basis of mtDNA barcoding are not always reflected in the nuclear genome, and advocate the use of AFLP markers as a check when mtDNA barcoding gives unexpected results.

Original language	English
Pages (from-to)	264-273
Number of pages	10
Journal	Molecular ecology resources
Volume	10
Issue number	2
DOIs	https://doi.org/10.1111/j.1755-0998.2009.02763.x
Publication status	Published - Mar 2010

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title = "Mitochondrial DNA barcoding detects some species that are real, and some that are not",

abstract = "Mimicry and extensive geographical subspecies polymorphism combine to make species in the ithomiine butterfly genus Mechanitis (Lepidoptera; Nymphalidae) difficult to determine. We use mitochondrial DNA (mtDNA) barcoding, nuclear sequences and amplified fragment length polymorphism (AFLP) genotyping to investigate species limits in this genus. Although earlier biosystematic studies based on morphology described only four species, mtDNA barcoding revealed eight well-differentiated haplogroups, suggesting the presence of four new putative 'cryptic species'. However, AFLP markers supported only one of these four new 'cryptic species' as biologically meaningful. We demonstrate that in this genus, deep genetic divisions expected on the basis of mtDNA barcoding are not always reflected in the nuclear genome, and advocate the use of AFLP markers as a check when mtDNA barcoding gives unexpected results.",

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AU - Dasmahapatra, Kanchon K.

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AU - Hoffman, Joseph I.

AU - Mallet, James

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Mitochondrial DNA barcoding detects some species that are real, and some that are not

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