By the same authors

Modeling of DNA replication in rapidly growing bacteria with one and two replication origins

Research output: Working paper

Standard

Modeling of DNA replication in rapidly growing bacteria with one and two replication origins. / Hawkins, Michelle Sarah; Retkute, Renata; Nieduszynski, Conrad; Rudolph, Christian.

2018.

Research output: Working paper

Harvard

Hawkins, MS, Retkute, R, Nieduszynski, C & Rudolph, C 2018 'Modeling of DNA replication in rapidly growing bacteria with one and two replication origins'. https://doi.org/10.1101/354654

APA

Hawkins, M. S., Retkute, R., Nieduszynski, C., & Rudolph, C. (2018). Modeling of DNA replication in rapidly growing bacteria with one and two replication origins. https://doi.org/10.1101/354654

Vancouver

Hawkins MS, Retkute R, Nieduszynski C, Rudolph C. Modeling of DNA replication in rapidly growing bacteria with one and two replication origins. 2018 Jun 24. https://doi.org/10.1101/354654

Author

Hawkins, Michelle Sarah ; Retkute, Renata ; Nieduszynski, Conrad ; Rudolph, Christian. / Modeling of DNA replication in rapidly growing bacteria with one and two replication origins. 2018.

Bibtex - Download

@techreport{72c25e4201d2430da875e12fa2b7d930,
title = "Modeling of DNA replication in rapidly growing bacteria with one and two replication origins",
abstract = "In rapidly growing bacteria initiation of DNA replication occurs at intervals shorter than the time required for completing genome duplication, leading to overlapping rounds of replication. We propose a mathematical model of DNA replication defined by the periodicity of replication initiation. Our model predicts that a steeper gradient of the replication profile is to be expected in origin proximal regions due to the overlapping rounds of synthesis. By comparing our model with experimental data from a strain with an additional replication origin, we predict defined alterations to replication parameters: (i) a reduced fork velocity when there were twice as many forks as normal; (ii) a slower fork speed if forks move in a direction opposite to normal, in line with head-on replication-transcription collisions being a major obstacle for fork progression; (iii) slower cell doubling for a double origin strain compared to wild-type cells; and (iv) potentially an earlier initiation of replication at the ectopic origin than at the natural origin, which, however, does not affect the overall time required to complete synthesis.",
author = "Hawkins, {Michelle Sarah} and Renata Retkute and Conrad Nieduszynski and Christian Rudolph",
year = "2018",
month = jun,
day = "24",
doi = "10.1101/354654",
language = "English",
type = "WorkingPaper",

}

RIS (suitable for import to EndNote) - Download

TY - UNPB

T1 - Modeling of DNA replication in rapidly growing bacteria with one and two replication origins

AU - Hawkins, Michelle Sarah

AU - Retkute, Renata

AU - Nieduszynski, Conrad

AU - Rudolph, Christian

PY - 2018/6/24

Y1 - 2018/6/24

N2 - In rapidly growing bacteria initiation of DNA replication occurs at intervals shorter than the time required for completing genome duplication, leading to overlapping rounds of replication. We propose a mathematical model of DNA replication defined by the periodicity of replication initiation. Our model predicts that a steeper gradient of the replication profile is to be expected in origin proximal regions due to the overlapping rounds of synthesis. By comparing our model with experimental data from a strain with an additional replication origin, we predict defined alterations to replication parameters: (i) a reduced fork velocity when there were twice as many forks as normal; (ii) a slower fork speed if forks move in a direction opposite to normal, in line with head-on replication-transcription collisions being a major obstacle for fork progression; (iii) slower cell doubling for a double origin strain compared to wild-type cells; and (iv) potentially an earlier initiation of replication at the ectopic origin than at the natural origin, which, however, does not affect the overall time required to complete synthesis.

AB - In rapidly growing bacteria initiation of DNA replication occurs at intervals shorter than the time required for completing genome duplication, leading to overlapping rounds of replication. We propose a mathematical model of DNA replication defined by the periodicity of replication initiation. Our model predicts that a steeper gradient of the replication profile is to be expected in origin proximal regions due to the overlapping rounds of synthesis. By comparing our model with experimental data from a strain with an additional replication origin, we predict defined alterations to replication parameters: (i) a reduced fork velocity when there were twice as many forks as normal; (ii) a slower fork speed if forks move in a direction opposite to normal, in line with head-on replication-transcription collisions being a major obstacle for fork progression; (iii) slower cell doubling for a double origin strain compared to wild-type cells; and (iv) potentially an earlier initiation of replication at the ectopic origin than at the natural origin, which, however, does not affect the overall time required to complete synthesis.

U2 - 10.1101/354654

DO - 10.1101/354654

M3 - Working paper

BT - Modeling of DNA replication in rapidly growing bacteria with one and two replication origins

ER -