Molecular analysis of the interaction between HPV type 16 E6 and human E6-associated protein

P R Daniels; C M Sanders; P Coulson; N J Maitland

Molecular analysis of the interaction between HPV type 16 E6 and human E6-associated protein

P R Daniels, C M Sanders, P Coulson, N J Maitland

Research output: Contribution to journal › Article › peer-review

Abstract

The complex formed between the human papillomavirus type 16 E6 protein and human E6-associated protein, which combine to ubiquitylate and degrade p53, has been studied bg chemical crosslinking. Analysis of the interactions of proteins purified from Escherichia coli as wen as proteins expressed in insect cells indicates that, while E6 has the capacity to form dimers, E6 and E6-associated protein interact as two monomers to form a heterologous dimer. (C) 1997 Federation of European Biochemical Societies.

Original language	English
Pages (from-to)	610
Number of pages	5
Journal	FEBS Letters
Volume	416
Issue number	1
Publication status	Published - 13 Oct 1997

Keywords

human papillomavirus
E6
E6-associated protein
crosslinking
HUMAN PAPILLOMAVIRUS TYPE-16
CERVICAL-CARCINOMA
CELL-LINES
P53
EXPRESSION
CANCER
DNA
ASSOCIATION
ONCOPROTEIN
DEGRADATION

Cite this

@article{57d923cfa3714ea1ad300bf4f6d21297,

title = "Molecular analysis of the interaction between HPV type 16 E6 and human E6-associated protein",

abstract = "The complex formed between the human papillomavirus type 16 E6 protein and human E6-associated protein, which combine to ubiquitylate and degrade p53, has been studied bg chemical crosslinking. Analysis of the interactions of proteins purified from Escherichia coli as wen as proteins expressed in insect cells indicates that, while E6 has the capacity to form dimers, E6 and E6-associated protein interact as two monomers to form a heterologous dimer. (C) 1997 Federation of European Biochemical Societies.",

keywords = "human papillomavirus, E6, E6-associated protein, crosslinking, HUMAN PAPILLOMAVIRUS TYPE-16, CERVICAL-CARCINOMA, CELL-LINES, P53, EXPRESSION, CANCER, DNA, ASSOCIATION, ONCOPROTEIN, DEGRADATION",

author = "Daniels, {P R} and Sanders, {C M} and P Coulson and Maitland, {N J}",

year = "1997",

month = oct,

day = "13",

language = "English",

volume = "416",

pages = "610",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Wiley-Blackwell",

number = "1",

}

TY - JOUR

T1 - Molecular analysis of the interaction between HPV type 16 E6 and human E6-associated protein

AU - Daniels, P R

AU - Sanders, C M

AU - Coulson, P

AU - Maitland, N J

PY - 1997/10/13

Y1 - 1997/10/13

N2 - The complex formed between the human papillomavirus type 16 E6 protein and human E6-associated protein, which combine to ubiquitylate and degrade p53, has been studied bg chemical crosslinking. Analysis of the interactions of proteins purified from Escherichia coli as wen as proteins expressed in insect cells indicates that, while E6 has the capacity to form dimers, E6 and E6-associated protein interact as two monomers to form a heterologous dimer. (C) 1997 Federation of European Biochemical Societies.

AB - The complex formed between the human papillomavirus type 16 E6 protein and human E6-associated protein, which combine to ubiquitylate and degrade p53, has been studied bg chemical crosslinking. Analysis of the interactions of proteins purified from Escherichia coli as wen as proteins expressed in insect cells indicates that, while E6 has the capacity to form dimers, E6 and E6-associated protein interact as two monomers to form a heterologous dimer. (C) 1997 Federation of European Biochemical Societies.

KW - human papillomavirus

KW - E6

KW - E6-associated protein

KW - crosslinking

KW - HUMAN PAPILLOMAVIRUS TYPE-16

KW - CERVICAL-CARCINOMA

KW - CELL-LINES

KW - P53

KW - EXPRESSION

KW - CANCER

KW - DNA

KW - ASSOCIATION

KW - ONCOPROTEIN

KW - DEGRADATION

M3 - Article

VL - 416

SP - 610

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 1

ER -