Molecular crowding in single eukaryotic cells: using cell environment biosensing and single-molecule optical microscopy to probe dependence on extracellular ionic strength, local glucose conditions, and sensor copy number

Jack Shepherd; Sarah Lecinski; Jasmine  Wragg; Sviatlana Shashkova; Chris MacDonald; Mark Christian Leake

doi:10.1016/j.ymeth.2020.10.015

Molecular crowding in single eukaryotic cells: using cell environment biosensing and single-molecule optical microscopy to probe dependence on extracellular ionic strength, local glucose conditions, and sensor copy number

Jack Shepherd, Sarah Lecinski, Jasmine Wragg, Sviatlana Shashkova, Chris MacDonald, Mark Christian Leake

Research output: Contribution to journal › Article › peer-review

Original language	English
Pages (from-to)	54-61
Number of pages	8
Journal	Methods
Volume	193
Early online date	4 Nov 2020
DOIs	https://doi.org/10.1016/j.ymeth.2020.10.015
Publication status	Published - 1 Sept 2021

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10.1016/j.ymeth.2020.10.015

Molecular crowding in yeast post review v8
© 2020 Elsevier Inc. This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy.
Accepted author manuscript, 1.87 MBLicence: CC BY-NC-ND

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Shepherd, J., Lecinski, S., Wragg, J., Shashkova, S., MacDonald, C., & Leake, M. C. (2021). Molecular crowding in single eukaryotic cells: using cell environment biosensing and single-molecule optical microscopy to probe dependence on extracellular ionic strength, local glucose conditions, and sensor copy number. Methods, 193, 54-61. https://doi.org/10.1016/j.ymeth.2020.10.015

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title = "Molecular crowding in single eukaryotic cells: using cell environment biosensing and single-molecule optical microscopy to probe dependence on extracellular ionic strength, local glucose conditions, and sensor copy number",

author = "Jack Shepherd and Sarah Lecinski and Jasmine Wragg and Sviatlana Shashkova and Chris MacDonald and Leake, {Mark Christian}",

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Shepherd, J , Lecinski, S, Wragg, J, Shashkova, S , MacDonald, C & Leake, MC 2021, 'Molecular crowding in single eukaryotic cells: using cell environment biosensing and single-molecule optical microscopy to probe dependence on extracellular ionic strength, local glucose conditions, and sensor copy number', Methods, vol. 193, pp. 54-61. https://doi.org/10.1016/j.ymeth.2020.10.015

Molecular crowding in single eukaryotic cells: using cell environment biosensing and single-molecule optical microscopy to probe dependence on extracellular ionic strength, local glucose conditions, and sensor copy number. / Shepherd, Jack ; Lecinski, Sarah; Wragg, Jasmine et al.
In: Methods, Vol. 193, 01.09.2021, p. 54-61.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Molecular crowding in single eukaryotic cells

T2 - using cell environment biosensing and single-molecule optical microscopy to probe dependence on extracellular ionic strength, local glucose conditions, and sensor copy number

AU - Shepherd, Jack

AU - Lecinski, Sarah

AU - Wragg, Jasmine

AU - Shashkova, Sviatlana

AU - MacDonald, Chris

AU - Leake, Mark Christian

PY - 2021/9/1

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U2 - 10.1016/j.ymeth.2020.10.015

DO - 10.1016/j.ymeth.2020.10.015

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JF - Methods

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Shepherd J , Lecinski S, Wragg J, Shashkova S , MacDonald C , Leake MC. Molecular crowding in single eukaryotic cells: using cell environment biosensing and single-molecule optical microscopy to probe dependence on extracellular ionic strength, local glucose conditions, and sensor copy number. Methods. 2021 Sept 1;193:54-61. Epub 2020 Nov 4. doi: 10.1016/j.ymeth.2020.10.015