Activities per year
Abstract
A Pb-mine site situated on acidic soil, but comprising of Ca-enriched islands around derelict buildings was used to study the spatial pattern of genetic diversity in Lumbricus rubellus. Two distinct genetic lineages ('A' and 'B'), differentiated at both the mitochondrial (mtDNA COII) and nuclear level (AFLPs) were revealed with a mean inter-lineage mtDNA sequence divergence of approximately 13%, indicative of a cryptic species complex. AFLP analysis indicates that lineage A individuals within one central 'ecological island' site are uniquely clustered, with little genetic overlap with lineage A individuals at the two peripheral sites. FTIR microspectroscopy of Pb-sequestering chloragocytes revealed different phosphate profiles in residents of adjacent acidic and calcareous islands. Bioinformatics found over-representation of Ca pathway genes in ESTPb libraries. Subsequent sequencing of a Ca-transport gene. SERCA, revealed mutations in the protein's cytosolic domain. We recommend the mandatory genotyping of all individuals prior to field-based ecotoxicological assays, particularly those using discriminating genomic technologies. (C) 2009 Elsevier Ltd. All rights reserved
Original language | English |
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Pages (from-to) | 883-890 |
Number of pages | 8 |
Journal | Environmental Pollution |
Volume | 158 |
Issue number | 3 |
DOIs | |
Publication status | Published - Mar 2010 |
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Metal munching earthworms – evolution in action
Mark Edward Hodson (Speaker)
17 Sept 2017Activity: Talk or presentation › Public lecture
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Geochemistry of the Earth's Surface
Mark Edward Hodson (Keynote/plenary speaker)
18 Aug 2014 → 22 Aug 2014Activity: Talk or presentation › Invited talk
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Metal-munching earthworms - evolution in action
Mark Edward Hodson (Speaker)
8 Oct 2013Activity: Talk or presentation › Invited talk