TY - JOUR
T1 - Molecular MRI in the Earth's Magnetic Field Using Continuous Hyperpolarization of a Biomolecule in Water
AU - Rovedo, Philipp
AU - Knecht, Stephan
AU - Bäumlisberger, Tim
AU - Cremer, Anna Lena
AU - Duckett, Simon B.
AU - Mewis, Ryan E.
AU - Green, Gary G R
AU - Burns, Michael
AU - Rayner, Peter J.
AU - Leibfritz, Dieter
AU - Korvink, Jan G.
AU - Hennig, Jürgen
AU - Pütz, Gerhard
AU - Von Elverfeldt, Dominik
AU - Hövener, Jan Bernd
N1 - © 2016 American Chemical Society. This is an author-produced version of the published paper. Uploaded in accordance with the publisher’s self-archiving policy. Further copying may not be permitted; contact the publisher for details. Embargo period: 12 months.
PY - 2016/6/30
Y1 - 2016/6/30
N2 - In this work, we illustrate a method to continuously hyperpolarize a biomolecule, nicotinamide, in water using parahydrogen and signal amplification by reversible exchange (SABRE). Building on the preparation procedure described recently by Truong et al. [ J. Phys. Chem. B, 2014, 118, 13882-13889 ], aqueous solutions of nicotinamide and an Ir-IMes catalyst were prepared for low-field NMR and MRI. The 1H-polarization was continuously renewed and monitored by NMR experiments at 5.9 mT for more than 1000 s. The polarization achieved corresponds to that induced by a 46 T magnet (P = 1.6 × 10-4) or an enhancement of 104. The polarization persisted, although reduced, if cell culture medium (DPBS with Ca2+ and Mg2+) or human cells (HL-60) were added, but was no longer observable after the addition of human blood. Using a portable MRI unit, fast 1H-MRI was enabled by cycling the magnetic field between 5 mT and the Earth's field for hyperpolarization and imaging, respectively. A model describing the underlying spin physics was developed that revealed a polarization pattern depending on both contact time and magnetic field. Furthermore, the model predicts an opposite phase of the dihydrogen and substrate signal after one exchange, which is likely to result in the cancelation of some signal at low field.
AB - In this work, we illustrate a method to continuously hyperpolarize a biomolecule, nicotinamide, in water using parahydrogen and signal amplification by reversible exchange (SABRE). Building on the preparation procedure described recently by Truong et al. [ J. Phys. Chem. B, 2014, 118, 13882-13889 ], aqueous solutions of nicotinamide and an Ir-IMes catalyst were prepared for low-field NMR and MRI. The 1H-polarization was continuously renewed and monitored by NMR experiments at 5.9 mT for more than 1000 s. The polarization achieved corresponds to that induced by a 46 T magnet (P = 1.6 × 10-4) or an enhancement of 104. The polarization persisted, although reduced, if cell culture medium (DPBS with Ca2+ and Mg2+) or human cells (HL-60) were added, but was no longer observable after the addition of human blood. Using a portable MRI unit, fast 1H-MRI was enabled by cycling the magnetic field between 5 mT and the Earth's field for hyperpolarization and imaging, respectively. A model describing the underlying spin physics was developed that revealed a polarization pattern depending on both contact time and magnetic field. Furthermore, the model predicts an opposite phase of the dihydrogen and substrate signal after one exchange, which is likely to result in the cancelation of some signal at low field.
UR - http://www.scopus.com/inward/record.url?scp=84976908730&partnerID=8YFLogxK
U2 - 10.1021/acs.jpcb.6b02830
DO - 10.1021/acs.jpcb.6b02830
M3 - Article
AN - SCOPUS:84976908730
SN - 1520-6106
VL - 120
SP - 5670
EP - 5677
JO - Journal of Physical Chemistry B
JF - Journal of Physical Chemistry B
IS - 25
ER -