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From the same journal

Molecular techniques for dicistrovirus detection without RNA extraction or purification

Research output: Contribution to journalArticlepeer-review

Published copy (DOI)

Author(s)

  • Jailson F B Querido
  • Jon Agirre
  • Gerardo A Marti
  • Diego M A Guérin
  • Marcelo Sousa Silva

Department/unit(s)

Publication details

JournalBioMed Research International
DatePublished - 2013
Volume2013
Pages (from-to)218593
Original languageEnglish

Abstract

Dicistroviridae is a new family of small, nonenveloped, and +ssRNA viruses pathogenic to both beneficial arthropods and insect pests as well. Triatoma virus (TrV), a dicistrovirus, is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of Chagas disease. In this work, we report a single-step method to identify TrV, a dicistrovirus, isolated from fecal samples of triatomines. The identification method proved to be quite sensitive, even without the extraction and purification of RNA virus.

    Research areas

  • Animals, Dicistroviridae, Feces, Insect Viruses, RNA, Triatoma

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