Monodehydroascorbate reductase mediates TNT toxicity in plants

Emily J. Johnston, Elizabeth L. Rylott, Emily Beynon, Astrid Lorenz, Victor Chechik, Neil C. Bruce*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The explosive 2,4,6-trinitrotoluene (TNT) is a highly toxic and persistent environmental pollutant. Due to the scale of affected areas, one of the most cost-effective and environmentally friendly means of removing explosives pollution could be the use of plants. However, mechanisms of TNT phytotoxicity have been elusive. Here, we reveal that phytotoxicity is caused by reduction of TNT in the mitochondria, forming a nitro radical that reacts with atmospheric oxygen, generating reactive superoxide. The reaction is catalyzed by monodehydroascorbate reductase 6 (MDHAR6), with Arabidopsis deficient in MDHAR6 displaying enhanced TNT tolerance. This discovery will contribute toward the remediation of contaminated sites. Moreover, in an environment of increasing herbicide resistance, with a shortage in new herbicide classes, our findings reveal MDHAR6 as a valuable plant-specific target.

Original languageEnglish
Pages (from-to)1072-1075
Number of pages4
JournalScience
Volume349
Issue number6252
DOIs
Publication statusPublished - 4 Sep 2015

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