Multi-compound electrophoretic assays for tyramine oxidase with a UV area detector imaging multiple windows on a looped capillary

Pawel L. Urban; David M. Goodall; Alexandre Z. Carvalho; Edmund T. Bergstrom; Ann Van Schepdael; Neil C. Bruce

doi:10.1016/j.chroma.2008.06.003

Multi-compound electrophoretic assays for tyramine oxidase with a UV area detector imaging multiple windows on a looped capillary

Pawel L. Urban, David M. Goodall, Alexandre Z. Carvalho, Edmund T. Bergstrom, Ann Van Schepdael, Neil C. Bruce

CNAP

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Abstract

Active pixel sensor UV area imaging and capacitively coupled contactless conductivity detection have been applied in an electrophoretically mediated microanalysis (EMMA) assay for substrate specificity of tyramine oxidase (Arthrobacter sp.). Use of the UV area imaging detector to monitor four windows in a capillary with three loops provided intrinsic self-referencing for all species and identified tyramine and 2-phenethylamine as the only reactive components in a multi-compound mixture. Continuous engagement EMMA experiments showed significant benefits by comparison with plug-plug EMMA, improving sensitivity by extending enzyme-substrate interaction times and allowing measurement of time-dependent reaction in the substrate zones passing the four windows. (C) 2008 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	52-63
Number of pages	12
Journal	Journal of Chromatography A
Volume	1206
Issue number	1
DOIs	https://doi.org/10.1016/j.chroma.2008.06.003
Publication status	Published - 3 Oct 2008

Keywords

Biocatalysis
Capillary electrophoresis
On-capillary enzyme assay
Substrate specificity
Tyramine oxidase
UV area imaging
CONTACTLESS CONDUCTIVITY DETECTION
LINEAR PHOTODIODE-ARRAY
PLASMA AMINE OXIDASE
ACTIVE PIXEL SENSOR
MEDIATED MICROANALYSIS
SUBSTRATE-SPECIFICITY
ZONE-ELECTROPHORESIS
MICROCHIP ELECTROPHORESIS
FUNDAMENTAL-ASPECTS
COLORIMETRIC ASSAY

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10.1016/j.chroma.2008.06.003

http://www.sciencedirect.com/science/article/pii/S002196730801011X

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@article{5075b30a689046c0985c03dcbc431b40,

title = "Multi-compound electrophoretic assays for tyramine oxidase with a UV area detector imaging multiple windows on a looped capillary",

abstract = "Active pixel sensor UV area imaging and capacitively coupled contactless conductivity detection have been applied in an electrophoretically mediated microanalysis (EMMA) assay for substrate specificity of tyramine oxidase (Arthrobacter sp.). Use of the UV area imaging detector to monitor four windows in a capillary with three loops provided intrinsic self-referencing for all species and identified tyramine and 2-phenethylamine as the only reactive components in a multi-compound mixture. Continuous engagement EMMA experiments showed significant benefits by comparison with plug-plug EMMA, improving sensitivity by extending enzyme-substrate interaction times and allowing measurement of time-dependent reaction in the substrate zones passing the four windows. (C) 2008 Elsevier B.V. All rights reserved.",

keywords = "Biocatalysis, Capillary electrophoresis, On-capillary enzyme assay, Substrate specificity, Tyramine oxidase, UV area imaging, CONTACTLESS CONDUCTIVITY DETECTION, LINEAR PHOTODIODE-ARRAY, PLASMA AMINE OXIDASE, ACTIVE PIXEL SENSOR, MEDIATED MICROANALYSIS, SUBSTRATE-SPECIFICITY, ZONE-ELECTROPHORESIS, MICROCHIP ELECTROPHORESIS, FUNDAMENTAL-ASPECTS, COLORIMETRIC ASSAY",

author = "Urban, {Pawel L.} and Goodall, {David M.} and Carvalho, {Alexandre Z.} and Bergstrom, {Edmund T.} and {Van Schepdael}, Ann and Bruce, {Neil C.}",

year = "2008",

month = oct,

day = "3",

doi = "10.1016/j.chroma.2008.06.003",

language = "English",

volume = "1206",

pages = "52--63",

journal = "Journal of Chromatography A",

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publisher = "Elsevier",

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T1 - Multi-compound electrophoretic assays for tyramine oxidase with a UV area detector imaging multiple windows on a looped capillary

AU - Urban, Pawel L.

AU - Goodall, David M.

AU - Carvalho, Alexandre Z.

AU - Bergstrom, Edmund T.

AU - Van Schepdael, Ann

AU - Bruce, Neil C.

PY - 2008/10/3

Y1 - 2008/10/3

N2 - Active pixel sensor UV area imaging and capacitively coupled contactless conductivity detection have been applied in an electrophoretically mediated microanalysis (EMMA) assay for substrate specificity of tyramine oxidase (Arthrobacter sp.). Use of the UV area imaging detector to monitor four windows in a capillary with three loops provided intrinsic self-referencing for all species and identified tyramine and 2-phenethylamine as the only reactive components in a multi-compound mixture. Continuous engagement EMMA experiments showed significant benefits by comparison with plug-plug EMMA, improving sensitivity by extending enzyme-substrate interaction times and allowing measurement of time-dependent reaction in the substrate zones passing the four windows. (C) 2008 Elsevier B.V. All rights reserved.

AB - Active pixel sensor UV area imaging and capacitively coupled contactless conductivity detection have been applied in an electrophoretically mediated microanalysis (EMMA) assay for substrate specificity of tyramine oxidase (Arthrobacter sp.). Use of the UV area imaging detector to monitor four windows in a capillary with three loops provided intrinsic self-referencing for all species and identified tyramine and 2-phenethylamine as the only reactive components in a multi-compound mixture. Continuous engagement EMMA experiments showed significant benefits by comparison with plug-plug EMMA, improving sensitivity by extending enzyme-substrate interaction times and allowing measurement of time-dependent reaction in the substrate zones passing the four windows. (C) 2008 Elsevier B.V. All rights reserved.

KW - Biocatalysis

KW - Capillary electrophoresis

KW - On-capillary enzyme assay

KW - Substrate specificity

KW - Tyramine oxidase

KW - UV area imaging

KW - CONTACTLESS CONDUCTIVITY DETECTION

KW - LINEAR PHOTODIODE-ARRAY

KW - PLASMA AMINE OXIDASE

KW - ACTIVE PIXEL SENSOR

KW - MEDIATED MICROANALYSIS

KW - SUBSTRATE-SPECIFICITY

KW - ZONE-ELECTROPHORESIS

KW - MICROCHIP ELECTROPHORESIS

KW - FUNDAMENTAL-ASPECTS

KW - COLORIMETRIC ASSAY

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U2 - 10.1016/j.chroma.2008.06.003

DO - 10.1016/j.chroma.2008.06.003

M3 - Article

SN - 0021-9673

VL - 1206

SP - 52

EP - 63

JO - Journal of Chromatography A

JF - Journal of Chromatography A

IS - 1

ER -

Multi-compound electrophoretic assays for tyramine oxidase with a UV area detector imaging multiple windows on a looped capillary

Abstract

Keywords

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